Detection Limits Of Live Attenuated Poultry Viral Vaccine Testing Method For Detection Of Bacterial Contamination
Journal Title: Journal of Applied Veterinary Sciences - Year 2019, Vol 4, Issue 1
Abstract
A total number of 27 vaccine vials including 3 batches of 3 different types of live attenuated poultry viral vaccines (tissue culture Gumboro vaccine, egg adapted colored ILT vaccine and egg adapted noncolored ILT vaccine) were experimentally contaminated with 1,5,10 CFU of Salmonella typhimurium reference strain/dose then subjected for sterility testing as referenced in OIE. Further dilution was carried out of vaccines showing negative results. The results of the statical analysis showed that there is a significant difference between direct inoculation of the vaccines and their dilutions. It could be concluded that the applied testing method is appropriate for testing sterility of living attenuated poultry viral vaccines but testing of some of such vaccines needs further dilutions to obtain accurate results.
Authors and Affiliations
Hanan M. Ibrahim, Hanan A. Ahmed, Nourhan Nagy, Gina M. Mohamed, Shafai S. M. , Aly, A. M
Keywords
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- EP ID EP677349
- DOI 10.21608/JAVS.2019.62668
- Views 201
- Downloads 0
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