Detection of AmpC β-Lactamases among Gram Negative Clinical Isolates
Journal Title: INTERNATIONAL JOURNAL OF RECENT TRENDS IN SCIENCE AND TECHNOLOGY - Year 2014, Vol 9, Issue 3
Abstract
Background and Objectives: AmpC β-lactamases confer resistance to a wide variety of β-lactam antibiotics including cephamycins such as cefoxitin and cefotetan, narrow and broad spectrum cephalosporins, aztreonam and are poorly inhibited by β-lactamase inhibitors such as clavulanic acid. The present study was undertaken to determine the occurrence of AmpC enzyme producing Gram negative clinical isolates at Navodaya Medical College, Raichur.Materials and Methods: 200 non-repeat Gram negative clinical isolates from various samples were screened for AmpC β- lactamases by using Modified disc Approximation method. The “Screen positive” isolates were detected for AmpC β-lactamase by Modified three-dimensional enzyme extract test, AmpC disk test, and phenyl boronic acid test.Results: Out of the 200 Gram negative isolates, 87 (43.5%) isolates were screened to be positive and 10 (5%) isolates were inducible AmpC producers. Of these 97 isolates subjected to confirmatory tests, 74 (37%) were harbouring AmpC enzymes. Majority of AmpC positive isolates were E.coli (20.5%), K.pneumoniae (5.5%) and Acinetobacter spp. (3.5%).Conclusion: Phenyl boronic acid test is the simple and practical method for the detection of AmpC in routine clinical microbiology laboratories. Plasmid-mediated AmpC β-lactamases have been associated with false in vitro susceptibility to cephalosporins. Thus, the type of β-lactamase produced by the organism should be detected along with the antibiogram for appropriate treatment of the patient.
Authors and Affiliations
Vijaya Shivanna , Achut Rao
Keywords
Related Articles
- EP ID EP110397
- DOI -
- Views 120
- Downloads 0
Evaluation of preoperative and postoperative corneal astigmatism after pterygium excision surgery with limbal autoconjunctival graft
Objectives: To evaluate the preoperative and postoperative corneal astigmatism after pterygium excision surgery with limbal autoconjunctival graft. To study relation between pterygium size with corneal astigmatism.
Role of Amoxicillin, Azithromycin and Moxifloxacin in the empirical treatment of community acquired bacterial sinusitis – An open label randomized study
Background: The study was conducted to compare the safety and efficacy of Amoxicillin, Azithromycin and Moxifloxacin in the empirical treatment of acute bacterial sinusitis. Materials: Inthis open label randomized study,...
The impact of patient’s pre operative characteristics on post operative health status in THR patients
Aim: To observe quality of life in THR patients. Objectives: To observe the quality of life before THR To observe quality of life after THR. To compare pre operative and post operative quality of life in THR Material and...
Study of Surgical Intervention in Patient of Meningocele with Hydrocephalus: Simultaneous V/S Sequential Group
Background: Meningocele is sac protruding from the spinal column, Myelomeningocele is most severe type of spina bifida, which is commonly encountered by surgeon in new born due to failure of caudal neurulation. The...
A study of clinical profile of intestinal obstruction in pediatric patients at tertiary health care center
Introduction: Small-bowel obstruction is a common surgical emergency and is a frequently encountered problem in abdominal surgery Aims and Objectives: To Study Clinical Profile of intestinal obstruction in Pediatric pati...