Abstract

Aspergillosis has a significant health concern for poultry and human, it causes economic losses in poultry flocks and many pulmonary infections in human. So our study was conducted to detect the incidence of Aspergillus species in both chicks and farm workers in private poultry farm in Qena Governorate, Egypt. 29 out of 40 examined laryngeal swaps (72.5%) collected from chicks and 15 out of 40 sputum samples (37.5%) collected from farm workers were positive for Aspergillus species. A.flavus , A.fumigatus and A.niger were detected in 60, 10 and 30% of examined chicks samples, while 12.5, 15 and 30% of examined workers samples were positive for these species respectively, beside A.ochraceus which was isolated at a low percentage (5%). aspHS gene was detected in 75 and 33.3% of A.fumigatus strains isolated from chicks and workers respectively. The sequence of the detected aspHS gene in our isolates was similar to reference strains registered with Gene bank at the percentages of 100% and 99.4%. The zoonotic role of this gene during infection was reinforced after the identity was found between our isolates in the amplified part (isolate No. 2 obtained from chicks and isolate No. 9 obtained from workers).

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