Detection of bacterial pathogens in surgical site infections and their antibiotic sensitivity profile
Journal Title: International Journal of Medical Research & Health Sciences (IJMRHS) - Year 2016, Vol 5, Issue 5
Abstract
Surgical site infections considered as a major problem in health care centers, resulting in extended length of stay, substantial associated morbidity and mortality, and high excess hospital cost. Thirty wound swabs were collected from patients who had developed postoperative wound infections at Rafidia Hospital-Nablus, Palestine. Bacterial isolates were identified according to standard microbiological methods. Antibiotics susceptibility test was applied for all isolated bacterial species. ERIC-PCR was carried out to determine the identity between isolated clones. The results of this research showed that the prevalence of pathogens among surgical site infections was 56.7%, 30%, 6.7%, 3.3% and 3.3% for E. coli, S. aureus, Klebsiella sp., Enterobacter sp., and Acinatobacter sp., respectively. E. coli isolates showed high resistance against Nalidixic acid (88.2%), Trimethoprim/Sulfamethoxazole (76.5%), Tetracycline (70.6%), Norfloxacin (64.7%) and Ciprofloxacin (58.5%). S. aureus showed high resistance against Nalidixic acid (88.9%), Norfloxacin (77.8%), Amoxycillin/clavulanic acid (77.8%), Kanamycin (66.7%) and Ciprofloxacin (55.6%). Methicillin resistant S. aureus (MRSA) accounted for 33.3% of a total of S. aureus isolates. Resistant to 3 or more antibiotics were detected in 94.1% (16/17) and 77.8% (7/9) of E. coli and S. aureus isolates, respectively. ERIC-PCR typing E. coli and S. aureus isolates showed that each was consisted of 4 ERIC-PCR clusters at a 50% similarity level. Indistinguishable and closely related strains were detected for both microorganisms. Results of this study might be important in provoking awareness to postoperative wound infections and further studies are needed to identify other pathogens responsible for SSIs and the source of infections. Using effective antibiotic policy will restrict further spread of postoperative wound infections.
Authors and Affiliations
Ghaleb Adwan| Department of Biology and Biotechnology, An-Najah National University, Nablus, Palestine, Nael Abu Hasan| Department of Biology and Biotechnology, An-Najah National University, Nablus, Palestine, Ibrahim Sabra| Rafidia Hospital, Department of Gynecology, Dalia Sabra| Department of Biology and Biotechnology, An-Najah National University, Nablus, Palestine, Shorouq Al-butmah| Department of Biology and Biotechnology, An-Najah National University, Nablus, Palestine, Shorouq Odeh| Department of Biology and Biotechnology, An-Najah National University, Nablus, Palestine, Zeinab Abd Albake| Department of Biology and Biotechnology, An-Najah National University, Nablus, Palestine, Haneen Badran| Department of Biology and Biotechnology, An-Najah National University, Nablus, Palestine
Keywords
Related Articles
- EP ID EP11660
- DOI -
- Views 317
- Downloads 9
PROSTHETIC MANAGEMENT OF PATIENT WITH PAPILLON-LEFEVRE SYNDROME: A CLINICAL REPORT
Papillon Lefevre syndrome is a rare autosomal recessive genetic disorder, which is characterized by palmarplantar hyperkeratosis, with rapidly progressive periodontitis and premature loss of both deciduous and permanent...
EFFECTS OF PRANAYAMA ON GALVANIC SKIN RESISTANCE (GSR), PULSE, BLOOD PRESSURE IN PREHYPERTENSIVE PATIENTS (JNC 7) WHO ARE NOT ON TREATMENT
Background: Psychological stress, in this era of urbanization, has become a part and parcel of our lives and has lead to serious problem affecting different life situation and carries a wide range of health related diso...
ASSESSMENT OF PARENTAL UNDERSTANDING OF PAEDIATRIC MEDICAL PRESCRIPTIONS
Introduction:Medical prescriptions are bound to be misinterpreted by patients and pharmacists if not properly conveyed. Pediatric prescriptions differ from adult prescriptions having wide variation in doses and formula...
USE OF PROTON PUMP INHIBITORS IN GENERAL PRACTICE: IS IT RATIONALE?
Background: The incidence of improper use of PPIs varies from 40-70% in various studies. Initiation and the continuous use of these drugs without correct indications will result in significant cost to the patient. The p...
Effectiveness of Muscle Stretching Exercise on Quality of Life of Haemodialysis Patients
Chronic Kidney disease and end stage renal disease have become worldwide major public health problems. The prevalence of ESRD cases in India was 700,000 in 2004 and its incidence rate was 173 per100, 000 people these c...