Detection of bacterial pathogens in surgical site infections and their antibiotic sensitivity profile
Journal Title: International Journal of Medical Research & Health Sciences (IJMRHS) - Year 2016, Vol 5, Issue 5
Abstract
Surgical site infections considered as a major problem in health care centers, resulting in extended length of stay, substantial associated morbidity and mortality, and high excess hospital cost. Thirty wound swabs were collected from patients who had developed postoperative wound infections at Rafidia Hospital-Nablus, Palestine. Bacterial isolates were identified according to standard microbiological methods. Antibiotics susceptibility test was applied for all isolated bacterial species. ERIC-PCR was carried out to determine the identity between isolated clones. The results of this research showed that the prevalence of pathogens among surgical site infections was 56.7%, 30%, 6.7%, 3.3% and 3.3% for E. coli, S. aureus, Klebsiella sp., Enterobacter sp., and Acinatobacter sp., respectively. E. coli isolates showed high resistance against Nalidixic acid (88.2%), Trimethoprim/Sulfamethoxazole (76.5%), Tetracycline (70.6%), Norfloxacin (64.7%) and Ciprofloxacin (58.5%). S. aureus showed high resistance against Nalidixic acid (88.9%), Norfloxacin (77.8%), Amoxycillin/clavulanic acid (77.8%), Kanamycin (66.7%) and Ciprofloxacin (55.6%). Methicillin resistant S. aureus (MRSA) accounted for 33.3% of a total of S. aureus isolates. Resistant to 3 or more antibiotics were detected in 94.1% (16/17) and 77.8% (7/9) of E. coli and S. aureus isolates, respectively. ERIC-PCR typing E. coli and S. aureus isolates showed that each was consisted of 4 ERIC-PCR clusters at a 50% similarity level. Indistinguishable and closely related strains were detected for both microorganisms. Results of this study might be important in provoking awareness to postoperative wound infections and further studies are needed to identify other pathogens responsible for SSIs and the source of infections. Using effective antibiotic policy will restrict further spread of postoperative wound infections.
Authors and Affiliations
Ghaleb Adwan| Department of Biology and Biotechnology, An-Najah National University, Nablus, Palestine, Nael Abu Hasan| Department of Biology and Biotechnology, An-Najah National University, Nablus, Palestine, Ibrahim Sabra| Rafidia Hospital, Department of Gynecology, Dalia Sabra| Department of Biology and Biotechnology, An-Najah National University, Nablus, Palestine, Shorouq Al-butmah| Department of Biology and Biotechnology, An-Najah National University, Nablus, Palestine, Shorouq Odeh| Department of Biology and Biotechnology, An-Najah National University, Nablus, Palestine, Zeinab Abd Albake| Department of Biology and Biotechnology, An-Najah National University, Nablus, Palestine, Haneen Badran| Department of Biology and Biotechnology, An-Najah National University, Nablus, Palestine
Keywords
Related Articles
- EP ID EP11660
- DOI -
- Views 295
- Downloads 9
Combined Systolic Diastolic Hypertension among Adults in Saudi Arabia: Prevalence, Risk Factors and Predictors: Results of a National Survey
Objective: This study aims to determine the prevalence, risk factors, and predictors, of systolic diastolic hypertension among adults in the Kingdom of Saudi Arabia. Methodology: A community-based cross-sectional study...
The Effects of Smoking on the Prostates of Rats which are Exposed to Cigarette Smoke
Background: Smoking is a hallmark preventable health issue worldwide. The role of smoking in the aetiology of prostate cancers remains a lack of consensus. Methods and material: 100 male albino Wistar rats were used. Th...
A study of changing trends in obstructive airway diseases & associated co-morbidities
Obstructive airway diseases (OAD), includes diseases characterized by increased resistance to airflow as a result of airway obstruction or airway narrowing. Types of obstructive lung diseases are asthma, COPD, bronchiec...
EFFECTS OF PRANAYAMA ON GALVANIC SKIN RESISTANCE (GSR), PULSE, BLOOD PRESSURE IN PREHYPERTENSIVE PATIENTS (JNC 7) WHO ARE NOT ON TREATMENT
Background: Psychological stress, in this era of urbanization, has become a part and parcel of our lives and has lead to serious problem affecting different life situation and carries a wide range of health related diso...
ROLE OF DIETARY DIVERSITY IN ENSURING ADEQUATE HAEMATOLOGICAL STATUS DURING PREGNANCY
Introduction: Though nutrition is a key input to blood formation, little is known about the extent maternal dietary quality contributes to the haematological status of pregnant women in Northern Region of Ghana. Objec...