Detection of Biofilm Producing Staphylococci By Three Different Methods and Their Antimicrobial Susceptibility Pattern in Urinary Catheter Tip Isolates
Journal Title: International Journal of Medical Research Professionals - Year 2017, Vol 3, Issue 3
Abstract
Introduction: Biofilms are group of microorganisms encased in an exopolymeric coat. They have been associated with a variety of persistent infections that respond poorly to conventional antibiotics. Purpose: To evaluate three different methods for detection of biofilm formation in staphylococci. Methods: For detection of biofilm formation, 40 clinical isolates of staphylococcus spp. were screened by Congo red agar (CRA) methods, Tube methods (TM) and Tissue culture plate (TCP) methods. Results: Out of 40 Staphylococcus spp., 26 were coagulase negative staphylococci (CNS) and 14 were coagulase positive staphylococci (CPS). 57.6% of CNS and 50% of CPS were slime producers. 22 isolates were detected as slime producer by TCP method, 18 by TM and 12 by CRA method. High resistances to conventional antibiotics were shown by biofilm producers. Conclusion: The TCP method was found to be most sensitive, accurate and reproducible screening method for detection of biofilm formation by staphylococci and has advantage of being a quantitative model to study the adherence of staphylococci on bio-medical devices.
Authors and Affiliations
Vijay Laxmi Meena, Rajni Sharma
Keywords
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- EP ID EP548095
- DOI 10.21276/ijmrp.2017.3.3.049
- Views 85
- Downloads 0
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