Detection of Biofilm Producing Staphylococci By Three Different Methods and Their Antimicrobial Susceptibility Pattern in Urinary Catheter Tip Isolates
Journal Title: International Journal of Medical Research Professionals - Year 2017, Vol 3, Issue 3
Abstract
Introduction: Biofilms are group of microorganisms encased in an exopolymeric coat. They have been associated with a variety of persistent infections that respond poorly to conventional antibiotics. Purpose: To evaluate three different methods for detection of biofilm formation in staphylococci. Methods: For detection of biofilm formation, 40 clinical isolates of staphylococcus spp. were screened by Congo red agar (CRA) methods, Tube methods (TM) and Tissue culture plate (TCP) methods. Results: Out of 40 Staphylococcus spp., 26 were coagulase negative staphylococci (CNS) and 14 were coagulase positive staphylococci (CPS). 57.6% of CNS and 50% of CPS were slime producers. 22 isolates were detected as slime producer by TCP method, 18 by TM and 12 by CRA method. High resistances to conventional antibiotics were shown by biofilm producers. Conclusion: The TCP method was found to be most sensitive, accurate and reproducible screening method for detection of biofilm formation by staphylococci and has advantage of being a quantitative model to study the adherence of staphylococci on bio-medical devices.
Authors and Affiliations
Vijay Laxmi Meena, Rajni Sharma
Keywords
Related Articles
- EP ID EP548095
- DOI 10.21276/ijmrp.2017.3.3.049
- Views 64
- Downloads 0
To Study the Cardiovascular Manifestations of Hypothyroidism and Their Response to Levothyroxine in Hilly Region of Northern India
Background: Hypothyroidism is a syndrome characterized by clinical and biochemical manifestations of thyroid hormone deficiencies in target tissues. It has multi systemic involvement among which cardiovascular manifestat...
Estimation of Stature from the Hand Length in Population of Rajasthan
Background: Physical anthropology involves measurement of various physical parameters referred to as anthropometric parameters. Estimation of height from the skeletal remains like amputated limbs has obvious significance...
Compliance of Type 2 Diabetic Patients to Its Management: A Cross-Sectional Survey
Background: Despite the efficacy of hypoglycemic medications in controlling blood glucose level, several studies indicated poor levels of compliance with these medications. Objectives: To estimate compliance of type 2 di...
Labor and the Perinatal Outcome during the Course of Pregnancy in Women and Their Infants with Extrapulmonary Tuberculosis
Introduction: Tuberculosis (TB) is a disease that affects hundreds of millions of people across the world. However, the incidence in developed countries has decreased over the past decades causing physicians to become un...
Gingival Health and Plaque Removal Assessment by Neem Stick and Toothbrush: A Comparative Study
Introduction: Oral hygiene measures have been practiced by different populations and cultures in a different way around the world. In various parts of the world where tooth brushing by modern method is uncommon or not po...