DETECTION OF BIOFILM PRODUCTION IN BLOOD CULTURE ISOLATES OF STAPHYLOCOCCI
Journal Title: International Journal of Medical Research & Health Sciences (IJMRHS) - Year 2015, Vol 4, Issue 1
Abstract
Background: Biofilm producing bacteria which are inherently resistant to antibiotics and disinfectants are widely associated with implant associated infections. Staphylococcus is the most commonly associated pathogens with bloodstream infection. Aims: The current study was conducted to detect biofilm production in Staphylococci isolated from blood culture specimens. Materials and Methods: 70 clinically significant staphylococcal isolates from blood culture were screened for biofilm production by Tissue culture plate (TCP) method, Tube method (TM) and Congo red agar (CRA) method and their antibiotic susceptibility profile was studied. Results: 59 out of 70 staphylococcal isolates were positive by TCP, out of these 21.4% staphylococci were high biofilm producers, 62.8% staphylococci were moderate biofilm producers and 15.8% were non-biofilm producers. Maximum resistance was observed in biofilm producers to cotrimoxazole (74.5%) and erythromycin (62.7%) and none were resistant to vancomycin and linezolid. Out of total 59 biofilm producers, 20.3 % (12) were methicillin resistant and all these were S. aureus isolates. 19% (1) out of total 11 biofilm non-producers were methicillin resistant. Conclusion: Biofilm production was seen to be a major virulence factor in most of the staphylococcal isolates obtained from patients with signs and symptoms of septicaemia. S. aureus was found to be the major pathogen and timely detection of biofilm producing phenotype should be carried out using a simple and reproducible method, TCP which is both qualitative and quantitative.
Authors and Affiliations
Gupta Puja| Postgraduate student, Professor & Head Department of Microbiology, HIMS, Dehradun, Uttarakhand, India, Gupta Pratima| Professor and Head, Department of Microbiology, AIIMS, Rishikesh, Uttarakhand, India, Mittal Garima| Assistant Professor, 4Professor & Head Department of Microbiology, HIMS, Dehradun, Uttarakhand, India, Corresponding author email: garimamittal80@gmail.com, Agarwal RK| Professor & Head Department of Microbiology, HIMS, Dehradun, Uttarakhand, India, Goyal Rohit| Assistant Professor, Department of Anaesthesiology, HIMS, Dehradun, Uttarakhand, India
Keywords
Related Articles
- EP ID EP11338
- DOI 10.5958/2319-5886.2015.00004.1
- Views 293
- Downloads 11
Tissue Doppler Imaging in Cardiology Nowadays: Clinical Applications
Echocardiography is a routine test in cardiology nowadays and Tissue Doppler imaging (TDI) is particularly useful for evaluating myocardial tissue motion; compared to strain imaging, TDI has a higher temporal resolution...
STRONGYLOIDES STERCORALIS HYPERINFECTION IN AN IATROGENICALLY IMMUNOSUPPRESSED PATIENT: A CASE REPORT
We report a case of Strongyloides stercoralis infection of the stomach in an elderly male patient who was on corticosteroids for six months. The patient presented with epigastric pain associated loss of appetite and lo...
COMPARISON OF POTENCY OF ANTIFUNGAL ACTION OF DANDRUFF SHAMPOOS AND DIFFERENT PLANT EXTRACTS
Context: Dandruff a very common scalp disorder with high prevalence in population is caused by numerous host factors in conjunction with Malassezia furfur. Most of the commercially available anti-dandruff hair shampoos...
Impending cardiogenic shock due to tricyclic antidepressant toxicity, computed tomography findings; a case report
A computed tomographic (CT) with contrast enhancement is extensively used for assessment of a wide range of thoracic and abdominal circumstances. Thus, as the use of CT in the evaluation of unstable patients increases,...
Evaluation of a carbon paste electrode modified with Strontium substituted bismuth and titanium oxide nanoparticles in the toxic metal chromium (VI) determination potentiometric method
Strontium substituted bismuth and titanium oxide nanoparticles with aurivillius morphology synthesized by chemical co-precipitation method and were characterized using XRD. The nanopartcles were used in the composition...