Detection of blaIMP gene encoding metallo-beta-lactamase resistance among clinical isolates of Pseudomonas aeruginosa
Journal Title: National Journal of Physiology, Pharmacy and Pharmacology - Year 2018, Vol 8, Issue 6
Abstract
Background: In recent decades, Pseudomonas aeruginosa has emerged as a multidrug-resistant bacteria by acquiring intrinsic resistance to a number of antimicrobial agents by uses distinctive resistant mechanisms to all the available antibiotics, which include metallo-β-lactamases (MBL) production, extended spectrum β-lactamase production, Amp C production, decreased permeability, altered penicillin binding proteins and rarely, and over expression of efflux pumps. Aims and Objectives: The aim of the study was to know the presence of IMP gene among P. aeruginosa isolates and to determine the carbapenem resistance mechanisms in carbapenem-resistant isolates of P. aeruginosa collected from sputum, blood, urine, pus, in Chennai, India. Materials and Methods: A total of 20 of non-repetitive clinical isolates of P. aeruginosa were collected and processed for biochemical tests and confirmed. Antibiotic susceptibility testing was determined by Kirby Bauer disc diffusion method. P. aeruginosa isolates were detected for the presence of blaIMP gene by polymerase chain reaction analysis. Results: Of the 20 clinical isolates of P. aeruginosa, 9/20 (45%) isolates were from sputum, 5/20 (25%) from blood, 3/20 (15%) from urine, and 3/20 (15%) from pus. Only 2/20 (10%) isolates showed sensitivity to imipenem. Other than that, for all other antibiotics isolates showed complete resistance 20/20 (100%). 17/20 (85%) clinical isolate of P. aeruginosa was found to possess blaIMP gene. Conclusion: The early detection of MBL‑producing P. aeruginosa may help in appropriate antimicrobial therapy and avoid the development and dissemination of these multidrug-resistant strains. However, to derive a conclusion, a more number of isolates are recommended and even other types of genes are also screened.
Authors and Affiliations
Varshan Raj, Gopinath Pa, Ashish R Jain
Keywords
Related Articles
- EP ID EP489598
- DOI 10.5455/njppp.2018.8.114549012018
- Views 51
- Downloads 0
Antimicrobial lotion containing red Piper betle leaf (Piper crocatum Ruiz and Pav) ethanolic extract for topical application
Background: Empirically red Piper betle leaf (Piper crocatum Ruiz and Pav) is used as a natural antiseptic, and it is proved that the ethanol extract of red P. betle leaf ethanol extract provides antimicrobial activity a...
Effect of Ehretia microphylla on the blood cholesterol and weight of ICR mice (Mus musculus)
Background: Simvastatin has been widely used in controlling blood cholesterol levels and weight; however, side effects such as muscle pain, muscle damage, liver damage, and hyperglycemia may occur. Studies have shown tha...
Adverse drug reactions: Two years' experience from a tertiary teaching hospital in Kerala
Background: Adverse drug reactions (ADRs) have become a major clinical problem causing additional burden on the total cost of a patient’s treatment. Aims and Objectives: To determine the characteristics of ADRs reported...
Comparative study on the effect of yogic relaxing asanas and pranayamas on cardiovascular response in healthy young volunteers
Background: Cardiovascular morbidity is increasing recently in India. Stress and autonomic dysfunction are associated with cardiovascular morbidity. Yoga is the best lifestyle ever designed. Based on limited scientific r...
Cyclic fluctuation of bleeding time and clotting time in various phases of menstrual cycle
Background: The physiologic mechanisms whereby the human endometrium maintains hemostasis during endovascular trophoblast invasion, yet permits menstrual hemorrhage, are unknown. Aims and Objective: To find out wh...