Detection of Carbapenem Resistance in Salmonella Species from a Tertiary Hospital in Eastern Cape, South Africa
Journal Title: Microbiology Research Journal International - Year 2015, Vol 10, Issue 3
Abstract
Aims: Broad-spectrum carbapenem group is the current therapy for strains of Enterobacteriaceae that express extended spectrum beta-lactamases (ESBLs). However, recent reports of therapeutic failures of carbapenems with strains that produce multiple β-lactamases are being documented. This study profiled antibiotic resistance in clinical isolates of Salmonella species in a tertiary hospital in the Eastern Cape of South Africa with the aim of identifying the status of Salmonella therapy in the region. Study Design: This is an analytical study. Place and Duration of Study: Salmonella isolates (119) from 96 blood and 23 stool specimens of patients attending Nelson Mandela Academic Hospital Complex (NMAHC) and surrounding clinics, Eastern Cape, South Africa collected for surveillance purposes over a period of 3 years (2006 – 2009) were obtained from National Institute of Communicable Diseases, (NICD), for analysis between 2010 and 2011. Methodology: Preliminary identification and serotyping were done at the NICD. The identification and antimicrobial susceptibility profile of isolates were confirmed with an Autoscan-4 antimicrobial susceptibility system. The MIC of ertapenem and imipenem tested for all Salmonella spp. were ≥2 mg/L and ≥4 mg/L respectively. Results: A considerable portion of the isolates 59/119 (49.6%) showed pentavalent resistance to some antibiotics including ampicillin and amoxicillin. Of the 59 multiply resistant isolates, 14 (23.7%) were resistant to 1 or more of the carbapenems examined. The phenotypic determination of ESBLs resulted in 25 (21.0%) ESBL-positive Salmonella isolates. Using Fisher's exact test, the proportion of carbapenem resistance isolates was significant at P value 0.032. Conclusion: The growing resistance of Salmonella isolates to carbapenem drugs in this setting call for caution in usage since this is a pointer to fewer options in the choice of drugs for ESBL’s therapy. Contact precautions should be put in place to forestall further transmission.
Authors and Affiliations
M. A. Bisi-Johnson, C. L. Obi
Keywords
Related Articles
- EP ID EP354785
- DOI 10.9734/BMRJ/2015/18586
- Views 111
- Downloads 0
Seroprevalence of Hepatitis C Virus amongst Blood Donors in Parts of North Central Nigeria
Background: Hepatitis C virus is one of the blood borne transmissible infections of public health significance. It can easily be transmitted to a wider population through transfusion of contaminated blood or blood produc...
Unusual Oropharyngeal Asymptomatic Manifestations Caused by Atypical Pathogens Detected by PCR into Altered Ecosystems of an Infertile Couple
The young couple casually arrived at our observation presented two different and painless altered ecosystems. The female partner exhibited inflamed lingual surface, while the man manifested several genital ulceration are...
Carbapenem-hydrolyzing Oxacillinase Genes in Clinical Isolates of Acinetobacter baumannii
Aims: We aimed in this study to detect the prevalence of carbapenem-hydrolyzing oxacillinase genes among Acinetobacter baumannii clinical isolates recovered from Assiut University Hospitals, Egypt. Methods: The antimicr...
Molecular, Phenotypic Characterization, Antimicrobial Susceptibility Profile of Staphylococcal Strains Isolated from Preterm Neonates at Neonatal Intensive Care Unit with Clinical Characteristics and Risk Factor of these Cases
Aim of the Work: The aim of work is to study the prevalence of different staphylococcal species in preterm neonates with septicemia at neonatal intensive care unit over a period of one year and their antimicrobial suscep...
Anaerobic Digestion of Biodegradable Domestic Wastes by Microorganisms
The aim of this study was to demonstrate that biogas can be generated from biodegradable domestic wastes and to determine the bacterial succession involved in the anaerobic decomposition of the wastes. Ten kilogram (10 k...