Detection of Cytotoxin-Associated and Vacuolating Cytotoxin Genotypes of Helicobacter pylori in Patients with Peptic Inflammatory/Ulcerative Disorders
Journal Title: The Egyptian Journal of Hospital Medicine - Year 2018, Vol 71, Issue 6
Abstract
<span>Background: </span><span>Helicobacter pylori </span><span>is a Gram negative, spiral, rod-shaped, and flagellated bacteria that colonizes the human gastric mucosa and can cause a strong inflammatory state and lesions. However, genomic and phenotypic features of different strains allow the expression of virulence factors which enable some strains, rather than others, to cause disease</span><span>. Aims: </span><span>The aim of the present study was to evaluate the role of the cytotoxicity genes, </span><span>CagA </span><span>and </span><span>VacA</span><span>, of </span><span>H. pylori </span><span>in patients with peptic inflammatory/ulcerative disorders and correlate between different genotypes and peptic lesions. </span><span>Patients and methods: </span><span>Upper gastrointestinal endoscopy and histopathological examination of gastric biopsy samples were done for 112 patients complaining of upper gastrointestinal symptoms and clinically suspected to have </span><span>H. Pylori </span><span>infection. </span><span>CagA </span><span>and </span><span>VacA </span><span>genotyping by PCR were done for 50 </span><span>H. pylori </span><span>+ve patients (diagnosed by histopathology). </span><span>Results: </span><span>CagA </span><span>gene and </span><span>VacA </span><span>gene were detected in 42.0% and 70% of </span><span>H. pylori </span><span>+ve patients respectively. There was a significant positive correlation between </span><span>CagA </span><span>and duodenal erosion and ulceration visualized by endoscopy. There was also a positive correlation between </span><span>CagA </span><span>and gastric erosion and ulceration visualized by endoscopy, but it didn`t reach a significant level. There was also a significant positive correlation between the </span><span>VacA m1s1 </span><span>subtype and duodenal erosion and ulceration detected by endoscopy. </span><span>VacA m2s2 </span><span>was correlated to presence of both gastric erosions and ulcerations and presence of metaplasia and atrophy. There was only one </span><span>H. pylori </span><span>+ve patient with gastric cancer. This patient was positive for both </span><span>CagA </span><span>and </span><span>VacA m2s2. </span><span>Conclusions: </span><span>The </span><span>CagA </span><span>gene is associated with severe forms of gastric pathology (peptic ulcer disease "PUD" and precancerous gastric lesions) and the </span><span>VacA m2s2 </span><span>subtype is associated with variable forms of gastric pathology rather than other </span><span>VacA </span><span>gene subtypes. </span><span>Recommendations: </span><span>Genotyping for </span><span>VacA </span><span>and </span><span>CagA </span><span>of </span><span>H. pylori </span><span>infected patients is helpful to determine the patients at more risk. Further studies are needed to evaluate the virulence factors in </span><span>H. pylori </span><span>with emphasis on role of </span><span>CagA </span><span>and </span><span>VacA m2s2 </span><span>both in vivo and in vitro.<br /> </span>
Authors and Affiliations
Eman Helal
Keywords
Related Articles
- EP ID EP583979
- DOI -
- Views 90
- Downloads 0
Manual Placental Removal versus Cord Traction for Placental Delivery at Caesarean Section in Correlation to Blood Loss
<strong>Background: </strong>Delivery by cesarean section is one of the most commonly performed obstetrical operations all over the world, but it exposes women to the inherent risks of major abdominal surgery, e.g., inju...
The Impact of "CYP2C9" and "VKORC1" Genetic Polymorphism upon Oral Anticoagulation Requirements
Background: Polymorphisms in the gene encoding the cytochrome P-450 2C9 enzyme (CYP2C9) are known to contribute to variability in sensitivity to Marevan. CYP2C9 is the enzyme primarily responsible for the metabolic clear...
Attitude and practice regarding the use of multivitamins for hair fall: Cross sectional study in Saudi Arabia
Background: Despite the lack of evidence which support the hypothesis that usage of multivitamins is effective in preventing hair fall, their use is still prevailing. Although recent studies demonstrated interference of...
Patients Satisfaction after Endodontic Treatment in Saudi Arabia
<strong>Background: </strong>Dental anxiety and fear appear to vary according to the type of treatment. Endodontic treatments have been shown to cause higher levels of anxiety than restorative or prophylactic treatments....
Assessment of Pulmonary Embolism Severity and Scoring Using Multi Slice CT in Correlation with Clinical Presentation and Echocardiography
<span>Aim of the work</span><span>: The aim of this study is to evaluate the correlation between pulmonary embolism </span> <span>severity and scoring obtained using multi slice CT (MSCT) and the clinical presentations a...