Detection of Dengue NS1 by a Comparative Analysis of Panbio Elisa and Rapid Diagnostic Test
Journal Title: Scholars Journal of Applied Medical Sciences - Year 2017, Vol 5, Issue 3
Abstract
Abstract: Early diagnosis of Dengue, decrease the incidence of Dengue hemorrhagic fever, Dengue shock syndrome. With the quick diagnostic methods, initiating early treatment to dengue patients, can decrease the dengue mortality rate from 20-30% in severe cases to less than 1%. Here in this study we have tried to analyze the prevalence of dengue infection by identifying NS1 detection using Rapid test and ELISA. A Prospective study on Dengue NS1 detection was done for two months (October, November 2015) in the Department of Microbiology at Government Medical College, Anantapuramu. At Microbiology Laboratory, serum of all samples was assessed for NS1 detection using two tests (PANBIO ELISA test, J. Mitra Rapid Dengue test) simultaneously. All the results were analyzed, entered into spread excel sheet and were compared between two tests. Out of 885 serum samples collected from suspected cases of Dengue in and around Anantapuramu, 134 (15.1%) were positive for Panbio ELISA and by Rapid diagnostic test, 113 (12.7%) samples were positive. On assessment of sensitivity and specificity of two tests for detection of Dengue NS1, it was observed that there is a slight higher sensitivity and specificity using Panbio ELISA than Rapid Diagnostic Test kit. NS1 ELISA test takes several steps and more time. RDT's require very less time about 15-30 mins, single step procedure. Even though ELISA has superior performance than RDT. In countries with fewer infrastructures and in remote areas, RDT's are more useful for early diagnosis and management of dengue with less expertise within a short time. Keywords:Dengue, ELISA, NS1, Rapid Diagnostic Test kit
Authors and Affiliations
A. Paradesi Naidu, P. Venkateswarlu, S. U madevi, M. Sailaja, B. Praveena, A. Renuka devi
Keywords
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