Detection of ESBL & MBL producing E.Coli From Urine Samples In A Tertiary Care Hospital In Jaipur, Rajasthan
Journal Title: Scholars Journal of Applied Medical Sciences - Year 2017, Vol 5, Issue 4
Abstract
Abstract: Urinary tract infections typically occur when bacteria enter the urinary tract through the urethra and begin to multiply in the bladder. Although the urinary system is designed to keep out such microscopic invaders, these defenses sometimes fail. When that happens, bacteria may take hold and grow into a full-blown infection in the urinary tract. The most common UTIs occur mainly in women and affect the bladder and urethra.The objective is to isolate and identify E.coli from urine samples. To perform antibiotic sensitivity of E.coli by Kirby bauers disc diffusion method. To detect ESBL and MBL producing E.coli by phenotypic method and study their antibiotic sensitivity pattern so as to provide basic guideline in the treatment.Patients included in the study were given a sterile, dry, test tube and request for 10-20ml specimen. A mid-stream urine specimen (MSU) collected preferably prior to administering antibiotics. Uncentrifuged urine is inoculated on CLED agar Culture plates areincubated at 37◦C for 24 hours. Detail of methodology is mentioned in material and method section.Out of the 71 isolates of E. Coli 36 (50.70%) were ESBLs producing. Out of the 71 isolates of E. coli 5 (7.04%) were MBL producing. In ESBLs producers group maximum susceptibilityis seen to imipenem, meropenam, (100%, 91.66% respectively) followed by Cefoperazone salbactum (77.77%), Nitrofurantoin (77.77 %), Amikacin (58.33 %), Ceftazidime clavulanate (55.55 %), Piperacillin tazobactum (52.77%), Aztreonam (33.33%) followed by Cotrimoxazole (33.33%) and ceftazidime (00.00%). E.coli 71 (47.33%) was the most common organism isolated from urine samples followed by Klebsiella spp. 31(21.33%), Citrobacter spp.17 (11.33%), Pseudomonas spp.15 (10%), Aceinetobacter 5(3.33%), Proteus spp.4 (2.66%) Enterobacter spp.3 (2%) staphylococci 2(1.33%) and candida albicans 1(0.66%) was the least common organism isolated. Keywords:ESBL, MBL, E Coli
Authors and Affiliations
Kuldeep yadav, Nitika Sharma
Keywords
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