Detection of Foot and Mouth Disease Virus in clinical samples by PCR-Elisa
Journal Title: Scholars Journal of Applied Medical Sciences - Year 2015, Vol 3, Issue 8
Abstract
Foot-and-mouth disease (FMD) is one of the highly contagious diseases of domestic animals. Effective control of this disease needs sensitive, specific, and quick diagnostic tools. In this paper seventy suspected field's samples to FMD were analyzed using virus isolation on cell culture, Enzyme Linked Immunosorbant Assay (ELISA), RT-PCR (Reverse Transcription- Polymerase Chain Reaction) and PCR-ELISA. In RT-PCR and PCR-ELISA detection of conserved and universal region of the virus was detected. For this purpose annealing primer to 2B region of the FMDV genome was applied. In our study ELISA used as a golden test and sensitivity of the other test was calculated according this test. Sensitivity of virus isolation, RT-PCR and PCR-ELISA was determined 85.7%, 96.6% and 100% respectively. The highest dilution of FMDV A2005IR 105.7 TCID50/ml (Tissue Culture Infectious Dose 50%/ Milliliter) that can be detected by ELISA, RT-PCR and PCR-ELISA was found 1/100, 1/10, 1/1000 viral stock dilutions respectively. Specificity of primer and probe that used in RT-PCR and PCR-ELISA evaluated by intact bovine epithelial tissue and after the PCR cycles no amplification was detected. Keywords: PCR-ELISA, RT-PCR, Foot and Mouth Disease virus
Authors and Affiliations
S. M. Azimi, H. Mahravani, F. Jeirani
Keywords
Related Articles
- EP ID EP374530
- DOI -
- Views 37
- Downloads 0
A Study of Outcome of Foramen Magnum Decompression and Duraplasty for Chiari Malformations
Abstract:Chiari malformations appears to be complex in its presentation, cause and natural history. The true incidence is not known. Around 3% in children and 1% in adults demonstrate radiological evidence, and all cases...
A study of biochemical changes in gallstone disease among alcoholics in karaikal population
Abstract: Gallstones are the major cause of corbidity worldwide, Gallstones (cholelithiasis) develops because of an imbalance in the chemical composition of bile inside the gallbladder and it is due to multifactorial eti...
Role of Laparoscopy in Diagnosis of Patients with Chronic Pain Abdomen
Chronic abdominal pain is a common problem faced by the surgical specialist. It leads to physical and psychological disability in a person. Despite radiological and clinical investigations when diagnosis cannot be ascert...
Clinico Microbiological Spectrum of Diabetic Foot Infections
Abstract: Diabetic foot infections are a common cause of morbidity and mortality. This study was undertaken to study the microbiological flora of diabetic foot ulcers and to assess the in vitro antibacterial susceptibili...
A Study of Clinical Features and Surgical Outcome of Chiari Type-I Malformation Associated with Syringomyelia
The Chiari malformation type 1 (CM1) has been subject to a recent examination anatomically and clinically where caudal displacement of the hindbrain through the foramenmagnum more than 5mm. The advent of modern neuroimag...