Detection Of Fusarium Species That Produce Fumonisin B1 In Maize Kernels Using Molecular Methods
Journal Title: INTERNATIONAL JOURNAL OF CURRENT RESEARCH - Year 2015, Vol 7, Issue 7
Abstract
Eighty eight Maize grains samples were collected between November 2013 and May 2014 from local markets and silo of Baghdad city. The aim of study was to identification of Fusarium species which associated with maize kernels and their ability to produce FB1 by using traditional and molecular methods. The results revealed that eight fungal genera found in maize samples as follow: Aspergillus (58.6 %) was predominant fungus followed by Fusarium (17.5%) and then Rhizopus (6.8 %), Alternaria (6.1 %), Mucor and Penicillium (3.9 %), Bipolaris (1.9 %), and Trichothecium (1.2%). The predominant species among Fusarium genera was F. verticillioides (70.64%) followed by F. proliferatum (8.26%). Specie specific PCR reactions revealed that out of seventy seven isolates only thirteen isolates belong to F. verticillioides and out of nine isolates only one isolate belong to F. proliferatum. All the thirteen F. verticillioides isolates possess fum1 gene, while absent in F. proliferatum.
Authors and Affiliations
Sinai W. Mohammed . , Khalid A. Habib . , Salim R. Al-Obaidy .
Keywords
Related Articles
- EP ID EP405512
- DOI -
- Views 109
- Downloads 0
Right sided massive Haemorrhagic pancreatic pleural effusions
Pancreatic disorders like Acute pancreatitis, Pancreatic Abscess, Pseudocyst, Pancreatic malignancy, Chronic pancreatitis present with pleural effusions. Here we present four cases of right sided massive hemorrhagic pan...
Learning Idiosyncrasies Of The Business Students In The Selected Arab States
Learning idiosyncrasies which is interchangeably used to refer to learning preferences in this research is an important topic in education since students learning preference are part of their individuality, their idiosyn...
Over weight, obesity and awareness of long term risks among two high schools in khartoum state, sudan
Background: During the last 2 decades overweight and obesity have increased globally among children and adolescents. Methods: This was a descriptive, cross sectional study conducted during the period October 2012 to Janu...
Recent Trends In Complete Denture Fabrication Among Specialist Prosthodontists In India
Variations may be observed in material selection as well as techniques followed at every step in complete denture fabrication. This survey aims to identify materials and methods used by specialist prosthodontists working...
A study of risk factor associations and etiology of upper gastrointestinal bleed
Upper gastrointestinal bleed has varied etiology and clinical presentations ranging from single episode of malena to profound hypovolemic shock, requiring immediate fluid resuscitation and urgent upper gastrointestinal e...