Detection of Heavy Metal Resistance Genes in an Environmental Pseudomonas aeruginosa Isolate

Journal Title: Microbiology Research Journal International - Year 2016, Vol 17, Issue 1

Abstract

Aim: To detect heavy metals resistance genes in an environmental Pseudomonas aeruginoas (S 7) isolate. Place and Duration of Study: Department of Biology, Faculty of Applied Science, Umm Al-Qura University, Makkah, Saudi Arabia, between January and March 2016. Methodology: Detection of PcoR and CzcD genes involved in metal resistance by PCR. Cloning PCR products and insert the cloned genes into E. coli XL1-Blue. A PCR screening of the two genes was performed on E. coli XL1-Blue to confirm the detection of the two genes and finally amplified cloned PcoR and CzcD genes were sequenced for further confirmation. Results: Using primers PcoR and CzcD, two PCR products of 636 bp and 389 bp respectively were detected in P. aeruginosa (S 7). A PCR screening of E. coli XL1-Blue that served as host for the cloned PCR products gave a 636 bp amplification with primer PcoR and a 389 bp amplification with primer CzcD. The inserted genes in E. coli XL-1 Blue were sequenced and confirmed the presence of PcoR and CzcD genes. Conclusion: The Pseudomonas aeruginosa (S 7) isolate reported in this study that showed remarkable tolerance to heavy metals by possessing genes involved in two types of efflux systems (P-type ATPase and Cation Diffusion Facilitator), may requires further investigation for its genetic structure and capability to be used in bioremediation of metals-contaminated environments.

Authors and Affiliations

Alaa Mihdir, Abdulrahman S. A. Assaeedi, Hussein H. Abulreesh, Gamal E. H. Osman

Keywords

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  • EP ID EP350925
  • DOI 10.9734/BMRJ/2016/28655
  • Views 75
  • Downloads 0

How To Cite

Alaa Mihdir, Abdulrahman S. A. Assaeedi, Hussein H. Abulreesh, Gamal E. H. Osman (2016). Detection of Heavy Metal Resistance Genes in an Environmental Pseudomonas aeruginosa Isolate. Microbiology Research Journal International, 17(1), 1-9. https://europub.co.uk/articles/-A-350925