Detection of Heavy Metal Resistance Genes in an Environmental Pseudomonas aeruginosa Isolate
Journal Title: Microbiology Research Journal International - Year 2016, Vol 17, Issue 1
Abstract
Aim: To detect heavy metals resistance genes in an environmental Pseudomonas aeruginoas (S 7) isolate. Place and Duration of Study: Department of Biology, Faculty of Applied Science, Umm Al-Qura University, Makkah, Saudi Arabia, between January and March 2016. Methodology: Detection of PcoR and CzcD genes involved in metal resistance by PCR. Cloning PCR products and insert the cloned genes into E. coli XL1-Blue. A PCR screening of the two genes was performed on E. coli XL1-Blue to confirm the detection of the two genes and finally amplified cloned PcoR and CzcD genes were sequenced for further confirmation. Results: Using primers PcoR and CzcD, two PCR products of 636 bp and 389 bp respectively were detected in P. aeruginosa (S 7). A PCR screening of E. coli XL1-Blue that served as host for the cloned PCR products gave a 636 bp amplification with primer PcoR and a 389 bp amplification with primer CzcD. The inserted genes in E. coli XL-1 Blue were sequenced and confirmed the presence of PcoR and CzcD genes. Conclusion: The Pseudomonas aeruginosa (S 7) isolate reported in this study that showed remarkable tolerance to heavy metals by possessing genes involved in two types of efflux systems (P-type ATPase and Cation Diffusion Facilitator), may requires further investigation for its genetic structure and capability to be used in bioremediation of metals-contaminated environments.
Authors and Affiliations
Alaa Mihdir, Abdulrahman S. A. Assaeedi, Hussein H. Abulreesh, Gamal E. H. Osman
Keywords
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- EP ID EP350925
- DOI 10.9734/BMRJ/2016/28655
- Views 57
- Downloads 0
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