Detection of human papillomavirus DNA in oral inverted ductal papillomas
Journal Title: Clinical Molecular Pathology - Year 2003, Vol 56, Issue 12
Abstract
Additional sections were obtained for the purpose of HPV DNA hybridisation studies using commercially available probes. Initially, a wide spectrum biotinylated probe (Dako, Carpinteria, California, USA) for common HPV types was used, according to the manufacturer’s suggested protocol (Dako GenPoint K0620 catalysed signal amplification system for in situ hybridisation; Dako). The wide spectrum probe (Y1404) targets the genomic DNA of HPV types 6, 11, 16, 18, 30, 31, 33, 35, 45, 51, and 52. Further subtyping was carried out on cases found to be positive using the wide spectrum probe. Three type specific probes for HPV 6/11 (Y1411), HPV 31/33 (Y1413), and HPV 16/18 (Y1412) were used for subtyping.
Authors and Affiliations
C Haberland-Carrodeguas, M L Fornatora, R F Reich, P D Freedman
Keywords
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- EP ID EP679619
- DOI 10.1136/jcp.56.12.910
- Views 54
- Downloads 0
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