Detection of Hypervirulent Klebsiella pneumoniae in Tanta University Hospital, Egypt
Journal Title: Microbiology Research Journal International - Year 2016, Vol 17, Issue 2
Abstract
Aims: This study aimed to investigate the frequency of hypervirulent Klebsiella pneumoniae (K. pneumoniae) isolated from various hospital-acquired infection cases admitted to Tanta University Hospital, Egypt and to determine the antibiotic resistance profile of these isolates. Study Design: Retrospective observational study. Place and Duration of Study: After collection of K. pneumoniae isolates from microbiology laboratory of Tanta University Hospital. Further work was carried out in the laboratory of Microbiology and Immunology Department, Faculty of Medicine, Tanta University, Egypt, from June 2015 to May 2016. Methodology: A total of 113 K. pneumoniae isolates were collected from different hospital-acquired infections and were tested for hypermucoviscosity phenotype by string test. Antibiotic disc diffusion test was performed for all isolates to identify their resistance pattern. Existence of rmpA gene was investigated by polymerase chain reaction. Results: Forty-six out of 113 (40.71%) isolates were string test-positive (HVKP), the remaining 67 (59.29%) negative isolates were CKP. Twenty-six (56.52%) out of 46 HVKP isolates possessed rmpA gene. Lower resistance rates were observed in HVKP than CKP. Conclusion: ESBL production by rmpA-positive HVKP isolates in hospital-acquired infections is worrisome, though its rate is still low. Control of the spread of this organism in the hospital environment and the general community is an important concern.
Authors and Affiliations
Marwa M. E. Abd-Elmonsef, Haidy S. Khalil, Amal Selim, Sherief Abd-Elsalam, Walaa Elkhalawany, Sameh Samir, Mohamed S. Abd-Elghafar, Mohamed M. E. Abd-Elmonsef
Keywords
Related Articles
- EP ID EP350758
- DOI 10.9734/BMRJ/2016/29240
- Views 87
- Downloads 0
Antibacterial Screening of Two Indigenous Nigerian Spices; Piper guineense and Xylopia aethiopica
Leaf and fruit extracts of Piper guineense and Xylopia aethiopica were respectively evaluated for their antibacterial activities against two (2) organisms; Staphylococcus aureus and Pseudomonas aeruginosa. The antibacter...
Effects of PH and Storage Temperatures on Antibacterial Activity of Bacteriocin Produced by Lactic Acid Bacteria Isolated from OGI
Aims: Lactic acid bacteria are important organisms recognized for their fermentative ability. They produce various compounds including bacteriocins which are proteinaceous antimicrobial compounds. The purpose of this stu...
Comparative Study of Blood Culture and Widal Agglutination Test from the Patients Suspected of Enteric Fever
Aims: This study was performed to identify the enteric fever cases by both blood culture and Widal agglutination test and compare the results obtained from both methods. Study Design: This research was carried out as hos...
Cattle Urine as a Fertiliser: Micro-biochemical Changes in Fermenting Cattle Urine and Implications on Plant Nutrient Conservation
Aim: The aim of this study was to evaluate the microbial and biochemical changes in fermenting urine, a practice used by farmers in Sub-Saharan Africa before its application as a soil fertility input. Methodology: Two 5...
Effect of Inoculum Size and Culture Age on the Cellular Properties and Host-Pathogen Interactions of Cryptococcus neoformans
Aims: Cryptococcus neoformans is a fungal pathogen which infection caused devastating morbidity and mortality in immunocompromised patients. The present study investigated the effect of culture starting inoculum size and...