DETECTION OF METHICILLIN RESISTANT STAPHYLOCOCCUS AREUS ISOLATED FROM NASAL CARRIAGE OF HEALTH CARE WORKERS BY POLYMERASE CHAIN REACTION
Journal Title: Journal of Experimental Biology and Agricultural Sciences - Year 2017, Vol 5, Issue 0
Abstract
Methicillin resistant Staphylococcus aureus (MRSA) and coagulase negative Staphylococci (MRCoNS) aremajor health problems worldwide. There is a growing concern about the rapid rise in resistance of nosocomialinfections to antimicrobial agents. The aim of the present study was to determine nasal colonization of MRSAand MRCoNS among healthcare workers (HCWs) in King Abdulaziz University hospital. A total of 100respondents were selected for this study and were classified into two groups: HCWs group which included 50technicians and nurses, and the control group which included 50 university students. Nasal swabs from anteriornares were cultured on selective media for the presence of mecA gene that is common to these bacteria.Polymerase chain reaction (PCR) and specific primers were used to determine mecA and coagulase gene (coa).The identified isolates were tested by restriction fragment length polymorphism (RFLP). Results of currentstudy revealed that among 50 HCWs, 21 (42%) were nasal carriers for MRCoNS and 12 (24%) for MRSA.The control group was negative for MRSA and MRCoNS. The results of the current study will help in makingdecision to prevent the spread of infections among workers in the field of health care.
Authors and Affiliations
Seham O. Alsulami, Huda A. Al Doghaither, Archana P. Iyer
Effect of small molecule inhibitor fisetin over proliferation of human non-small cell pulmonary carcinoma
The percentage of cancer-related deaths attributable to diet and tobacco globally is reported to be as high as 60-70%. A large number of dietary compounds have gained research interest and tested to determine their chemo...
CRUDE GLYCEROL: BY-PRODUCT OF BIODIESEL INDUSTRIES AS AN ALTERNATIVE ENERGY SOURCE FOR LIVESTOCK FEEDING
Increased concern on greenhouse gas emission, concomitant global warming and decreasing fossil fuels have created the necessity to find an alternative source of energy for the transportation vehicles. Biodiesels is an al...
EFFECT OF PROBIOTIC AND PRILLED FAT ON LACTATION PERFORMANCE OF CROSS BRED COW DURING TRANSITION PERIOD
In present study, combined effects of prilled fat and yeast culture (YC) mixture supplemented diet on 12 early lactating crossbred cows were evaluated. Cows were divided into two groups as control group (CON group) and s...
SURFACE STERILIZATION PROTOCOL FOR Curcuma angustifolia ROXB. MICROPROPAGATION
For the micropropagation of Curcuma angustifolia usually rhizome buds are used as explants which are definitely associated with various types of soil-born microorganisms, therefore only one type of sterilant cannot be us...
The Role of Science and Technology in Enhancing Food Security in Arab Countries
The Arab world is the largest food deficit region in the world due to the major challenges facing increasing food production despite the high potential to increase agriculture production in most Arab countries. In 2013 t...