DETECTION OF METHICILLIN RESISTANT STAPHYLOCOCCUS AREUS ISOLATED FROM NASAL CARRIAGE OF HEALTH CARE WORKERS BY POLYMERASE CHAIN REACTION
Journal Title: Journal of Experimental Biology and Agricultural Sciences - Year 2017, Vol 5, Issue 0
Abstract
Methicillin resistant Staphylococcus aureus (MRSA) and coagulase negative Staphylococci (MRCoNS) aremajor health problems worldwide. There is a growing concern about the rapid rise in resistance of nosocomialinfections to antimicrobial agents. The aim of the present study was to determine nasal colonization of MRSAand MRCoNS among healthcare workers (HCWs) in King Abdulaziz University hospital. A total of 100respondents were selected for this study and were classified into two groups: HCWs group which included 50technicians and nurses, and the control group which included 50 university students. Nasal swabs from anteriornares were cultured on selective media for the presence of mecA gene that is common to these bacteria.Polymerase chain reaction (PCR) and specific primers were used to determine mecA and coagulase gene (coa).The identified isolates were tested by restriction fragment length polymorphism (RFLP). Results of currentstudy revealed that among 50 HCWs, 21 (42%) were nasal carriers for MRCoNS and 12 (24%) for MRSA.The control group was negative for MRSA and MRCoNS. The results of the current study will help in makingdecision to prevent the spread of infections among workers in the field of health care.
Authors and Affiliations
Seham O. Alsulami, Huda A. Al Doghaither, Archana P. Iyer
Keywords
Related Articles
- EP ID EP428022
- DOI 10.18006/2017.5(3).288.293
- Views 54
- Downloads 0
GGE BIPLOT ANALYSIS FOR YIELD PERFORMANCE OF GRAIN AMARANTH GENOTYPES ACROSS DIFFERENT ENVIRONMENTS IN WESTERN INDIA
Stable performance of grain amaranths genotypes at specific environment is critical for obtaining high and consistent yield. This study was conducted to assess grain yield stability of thirteen genotypes in varied enviro...
Soybean managing for maximize production: plant population density effects on seed yield and some agronomical traits in main cropped soybean production
Plant density is an essential agronomic practice and affects the productivity of crops. Hence, the optimum plant density to achieve the maximum productivity may vary from crop to crop, genotype to genotype and location t...
Effect of the interactions between moisture content, seed storage time and use of pesticides on seed germination, viability and viability indicators of wheat crop
In order to determine the effect of moisture content, storage time and pesticide (fungicide & insecticide) application in wheat variety Bezostaja -1, an experiment was conducted in Split-Split plot, randomized complete b...
CRUDE GLYCEROL: BY-PRODUCT OF BIODIESEL INDUSTRIES AS AN ALTERNATIVE ENERGY SOURCE FOR LIVESTOCK FEEDING
Increased concern on greenhouse gas emission, concomitant global warming and decreasing fossil fuels have created the necessity to find an alternative source of energy for the transportation vehicles. Biodiesels is an al...
An overview on the functional food concept: prospectives and applied researches in probiotics, prebiotics and synbiotics
The principal role of diet is to supply adequate nutrients providing energy to sustain physiologic functions and well-being. Every foods and feeds are functional and consumption of bioactive molecules is facilitated by d...