Detection of nivalenol and deoxynivalenol chemotypes produced by Fusarium graminearum species complex isolated from barley in Iran using specific PCR assays
Journal Title: Journal of Plant Protection Research - Year 2017, Vol 57, Issue 3
Abstract
In order to identify trichothecenes chemotypes produced by Fusarium graminearum species complex (FGSC) isolated from barley, 68 barley samples were collected from markets in Kermanshah and Hamedan provinces, Iran. Thirty-one Fusarium isolates were obtained from grains and morphologically classified into three species FGSC (14), F. equiseti (9), and F. proliferatum (8). The identification of the members of FGSC was confirmed molecularly using Fg16F/Fg16R primers. Fusarium asiaticum isolates (4) were distinguished from other FGSC using Fg6CTPSf177/Fg16R primers. Polymerase chain reaction-based (PCRbased) detection of mycotoxin-synthesis-pathway gene was also used to determine the potential of the analysed strains to produce deoxynivalenol (DON), 15-acetyldeoxynivalenol (15-AcDON), 3-acetyldeoxynivalenol (3-AcDON), and nivalenol (NIV). Of 14 tested isolates, 10 and 4 isolates belonged to DON and NIV chemotype, respectively. Also, the results of DON chemotype survey using specific primers MinusTri7F/R and Tri315F/R showed 1 and 9 isolates produced 3-AcDON and 15-AcDON, respectively. These results show that DON was the most common chemotype in western Iran. To our knowledge, this is the first report on 15-AcDON, 3-AcDON, and NIV isolated from barley in Iran.
Authors and Affiliations
Khosrow Chehri, Rasoul Godini
Keywords
Related Articles
- EP ID EP294968
- DOI 10.1515/jppr-2017-0033
- Views 80
- Downloads 0
Effects of loose kernel smut caused by Sporisorium cruentum on rhizomes of Sorghum halepense
The effect of loose kernel smut fungus Sporisorium cruentum on Sorghum halepense (Johnson grass) was investigated in vitro and in greenhouse experiments. Smut infection induced a decrease in the dry matter of rhizomes an...
Serological and molecular detection of Bean leaf roll and Chickpea chlorotic stunt luteoviruses in chickpea from Iran
Chickpea (Cicer arietinum L.) is an important legume crop and widely cultivated in northwestern provinces of Iran. During a survey in the 2015 growing season a total of 170 selected chickpea plants with general yellowing...
Rapid evolutionary dynamics of the Pepino mosaic virus – status and future perspectives
Pepino mosaic virus (PepMV) has emerged as an important pathogen of greenhouse tomato crops and is currently distributed worldwide. Population genetic studies have revealed a shift in the dominant PepMV genotype from Eur...
Effect of 1,8-cineol on the biology and physiology of elm leaf beetle, Xanthogaleruca luteola (Col.: Chrysomelidae)
The effect of monoterpenoid 1,8-cineol on the toxicity and physiology of elm leaf beetle, Xanthogaleruca luteola Müller under laboratory conditions (26 ± 1°C, 65 ± 10% RH and 16L : 8D h) was investigated. Initially, LC30...
Isolation, characterization and toxicity of native Bacillus thuringiensis isolates from different hosts and habitats in Iran
Bacillus thuringiensis is a Gram-positive, aerobic, facultative anaerobic and endosporeforming bacterium. Different strains of this species have the ability to produce parasporal crystalline inclusions which are toxic to...