Detection of Polioviruses in Sewage Using Cell Culture and Molecular Methods
Journal Title: Polish Journal of Microbiology - Year 2016, Vol 65, Issue 4
Abstract
The work presented here demonstrates the utility of a two-step algorithm for environmental poliovirus surveillance based on: preselection of sewage samples tested for the presence of enteroviral genetic material-RT-PCR assay and detection of infectious viruses by cell culture technique (L20B for polioviruses and RD for polio and other non-polio enteroviruses). RD and L20B cell lines were tested to determine their sensitivity for isolation of viruses from environmental samples (sewage). Finally, we wanted to determine if sewage concentration affects the results obtained for RT-PCR and cell cultures.
Authors and Affiliations
Agnieszka Figas, Magdalena Wieczorek, Bogumiła Litwińska, Włodzimierz Gut
Keywords
Related Articles
- EP ID EP258938
- DOI 10.5604/17331331.1227676
- Views 70
- Downloads 0
Investigation of the Actual Causes of Hip Joint Implant Loosening Classified as Aseptic – Analysis of Microbiological Culture Results and Levels of Inflammatory Markers
Loosening of the hip joint prosthesis is considered as one of the most significant postoperative complications in recent years. The laboratory diagnostic procedure used to differentiate periprosthetic infection from...
The Effect of Fe3O4 Nanoparticles on Survival of Probiotic Bacteria Lactobacillus acidophilus PCM2499 at Lower pH
This paper presents a description of an experiment in which the survival rate of the probiotic bacteria Lactobacillus acidophilus PCM2499 was increased only due to the presence of Fe3O4 magnetic nanoparticles. The surviv...
Comparison of Free and Immobilized L-asparaginase Synthesized by Gamma-Irradiated Penicillium cyclopium
Gamma irradiation is used on [i]Penicillium cyclopium[/i] in order to obtain mutant cells of high L-asparaginase productivity. Using gamma irradiation dose of 4 KGy, [i]P. cyclopium[/i] cells yielded L-asparaginase with...
N-Substituted N′-(2-alkylthio-4-chloro-5-methylbenzenesulfonyl) Guanidines – Antibacterial, Cytotoxic Activities and Theoretical Studies
A series of twenty nine [i]N[/i]-substituted [i]N′[/i]-(2-alkylthio-4-chloro-5-methylbenzenesulfonyl)guanidine derivatives bearing sulfonamide moiety have been screened [i]in vitro[/i] for antibacterial activity aga...
Bloodstream Infections due to Enterobacteriaceae Among Neonates in Poland – Molecular Analysis of the Isolates
Bloodstream infections (BSIs) are associated with a significantly increased risk of fatality. No report has been found about the molecular epidemiology of Enterobacteriaceae causing BSI in neonates in Poland. The aim of...