Detection of Prethrombin 1 in Human Blood Plasma
Journal Title: International Blood Research & Reviews - Year 2016, Vol 5, Issue 2
Abstract
Background: Prethrombin 1 is one of prothrombin derivatives which appears in the presence of thrombin in vitro. However, existence of prethrombin 1 in vivo remained questionable. The aim of present work was to detect of prethrombin 1 in vivo at abdominal aortic aneurysm, hip replacement after fracture and stroke. Methodology: Blood plasma samples of patients with abdominal aortic aneurysm, hip replacement after fracture, stroke, and patients which were treated with warfarine were collected. Detection of prethrombin 1 was completed using three independent approaches: combination of prothrombin index and ecamulin (ecarin) index; the APTT-test modified by addition of exogenous prothrombin; Western-blotting of blood plasma using polyclonal antibody to prothrombin. Results: Prethrombin 1 presence in patients’ blood plasma at studied pathologies was proven. Inactive prothrombin (descarboxy-prothrombin), but not prethrombin 1 was found in blood plasma of patients which were treated with warfarine. Developed approach can be used for testing the prethrombin 1 in clinical diagnostics. Conclusion: Prethrombin 1 at different pathologies, accompanied by coagulation disorders was detected in patients’ blood plasma directly.
Authors and Affiliations
Volodymyr Chernyshenko, Dar’ya Korolova, Tetiana Platonova, Tamara Chernyshenko, Eduard Lugovskoy
Keywords
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- EP ID EP242572
- DOI 10.9734/IBRR/2016/24683
- Views 147
- Downloads 0
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