Detection of Species-Specific Meat of Buffalo, Goat and Sheep by Multiplex-PCR Based Method
Journal Title: JOURNAL OF BIOLOGICAL ENGINEERING RESEARCH AND REVIEW - Year 2018, Vol 5, Issue 2
Abstract
The production of buffalo's, goat's, and sheep's meat has considerable economic importance based on their high consumption in various industries. It is commonly found that undesirable mixing in meat may lead to allergy, religious, ethical or cultural objections of undeclared meat during processing. In the present study, the polymerase chain reaction (PCR) has been validated to identify three meats (buffalo, sheep and goat) as raw materials for products simultaneously in one mixture. A forward primer and reverse primers were designed on a conserved DNA sequence in the mitochondrial cyt b gene and species -specific DNA sequences for each species respectively. Selected samples of meats from buffalo, goat and sheep were applied for molecular analysis after being critically characterized. Genomic DNA was isolated from each species in triplicates. The PCR products showed species -specific amplicons of 155 bp, 274 bp and 331 bp for goat, buffalo and sheep respectively. The method based on genetic traceability using PCR might become useful for the consumers, food industry, and law enforcement and applicable to different raw meats and their products.
Authors and Affiliations
Anupam Singh, Prachi Bhargava
Keywords
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