Detection of the polyphenolic components in[i] Ribes nigrum[/i] L.
Journal Title: Annals of Agricultural and Environmental Medicine - Year 2014, Vol 21, Issue 1
Abstract
Background. The blackcurrant ([i]Ribes nigrum [/i]L.) is a species of native currant which contains a lot of polyphenolic antioxidants which is used medicinally and has a fundamental role in the maintenance health.Materials, methods and objective. Ultraviolet–visible spectrophotometry and ultraviolet range high performance liquid chromatography (HPLC) were used to characterize the polyphenolic content of common Ribes nigrum collected in the western part of the Banat Region in Romania.Results. UV–visible spectrophotometry was a reliable tool for identifying the phenolic compounds class. Polyphenols calibration curves from the methanolic extracts showed a good linearity (r2>0.984) within test ranges and generated a well–designed absorption band with a local maximum at 273.2 nm band, which can be attributed to thr electronic transition of the n–p* type. Chromatographic separation and analysis of the methanol extract was useful for the structural epigallocatechin(EGC) and epigallocatechin–3–gallate (EGCG) characterization of primary antioxidant compounds.Conclusions. The new, slightly modified, chromatographic system can serve for the development of a quantitative assessment methodology of epigallocatechin and epigallocatechin–3–gallate compounds, as well as for the comparative characterisation mand standardisation of the dominant polyphenolic components in [i]Ribes nigrum[/i] using EGC and EGCG standards.
Authors and Affiliations
Monica BUTNARIU
Keywords
Related Articles
- EP ID EP80729
- DOI -
- Views 92
- Downloads 0
Andersen-Tawil syndrome (ATS) – Case report and literature review
Andersen-Tawil Syndrome (ATS) is a rare genetic disorder inherited in an autosomal dominant pattern caused by mutations in the [i]KCNJ2[/i] gene encoding Kir2.1 protein forming potassium ion channel, leading to disruptio...
Head of Polish society of Virology
A new concept for evaluating muscle function in the lower extremities in cases of low back pain syndrome in anamnesis
introduction. There are difficulties in objective evaluation of activity of the muscles in the lower extremities of patients after successful treatment of sciatica and pseudosciatica, when no clear clinical symptoms are...
Do inflammatory factors play a significant role in etiopathogenesis of endometrial cysts? Part 1
Endometriosis is an estrogen-related chronic condition which consists in the implantation and growth of endometrial cells outside the uterine cavity. It has an immune and inflammatory background, and to-date the precise...
Microflora and mycotoxin contamination in poultry feed mixtures from western Poland
Objective: Contamination of feeds with pathogenic microflora and mycotoxins constitutes a serious threat both for animals and humans. The aim of the study was to determine the degree of risk of the occurrence of microsco...