Detection of β-lactamase Mediated Resistance among Pseudomonas aeruginosa Isolates from Ocular Infections
Journal Title: National Journal of Laboratory Medicine - Year 2016, Vol 5, Issue 1
Abstract
Introduction: P.aeruginosa is the most prevalent gramnegative organism in ocular infections. Production of extended spectrum of b-lactamases (ESBL) by them may require alteration in the management protocol of gram-negative ocular infections. Aim: To analyze the prevalence and detection of β-lactamase producing P.aeruginosa from ocular infections. Materials and Methods: This study included all the patients with ocular infections who visited a tertiary care eye hospital in Coimbatore, for a period of 4 months. During this study period, the isolates were confirmed as P.aeruginosa by standard identification protocol. Minimum inhibitory concentration (MIC) of the antibioticswas determinedby agar dilution method. Inducible β-lactamases and extended spectrum β-lactamases were detected by double disc diffusion method. Results: Of the 2644 ocular specimens collected from 2618 patients, a total of 34 isolates of P.aeruginosa were obtained. MICs of cefotaxime and ceftazidime for all the isolates revealed that, 21 (62%) isolates were resistant to cefotaxime. A total of 11 strains were induced to produce β-lactamase with disc approximation at 20 mm apart and the presence of ESBL was detected in 4.3% of strains through 20 mm disc approximation, 34.7% of strains through 15 mm disc approximation. The synergic effect of cefotaxime with aminoglycosides was seen among 61.7% of strains. Conclusion: Resistance of P.aeruginosa to b-lactam antibiotics seems to be prevalent in this part of the state. Combination of cefotaxime with aminoglycosides may be more useful for treatment of severe eye infections caused by P. aeruginosa.
Authors and Affiliations
Palanisamy Manikandan
Keywords
Related Articles
- EP ID EP377408
- DOI 10.7860/NJLM/2016/14382:2089
- Views 112
- Downloads 0
Time and Motion Study of Blood Delivery System in a Regional Blood Transfusion Centre in West Bengal: A Pilot Study
Introduction: Delay in turnaround time (TAT) of blood delivery for transfusion is an important administrative problem in hospitals. Time and motion study for a definite root cause analysis (RCA) is valuable to identify t...
Adrenocortical Adenoma with Foci of Myelolipoma in a Patient of Conn’s Syndrome
Adrenal myelolipoma is an uncommon benign tumour usually discovered by chance in patients. It is usually inactive hormonally and composed of mature adipose tissue with normal haematopoietic cells. Rarely, adrenocortical...
Prevalence and Outcome of Clostridioides difficile Infection in a Tertiary Care Hospital in Kerala, India
ABSTRACT Introduction: Increasing incidence of Clostridioides difficile Infections (CDI) has been associated with antibiotic usage. The most commonly used test for its diagnosis is Glutamate Dehydrogenase (GDH) enzyme al...
Extended-Spectrum β-lactamase Production among Enterobacter cloacae and Enterobacter aerogenes at a Tertiary Care Center in Coastal Karnataka
Introduction: Enterobacter spp. have been increasingly isolated as nosocomial pathogens. Multi-drug resistance is more frequently noted among these pathogens. Extended spectrum beta lactamases (ESBLs) are difficult to de...
Sepsis with Spreading Retropharyngeal Abscess in a Diabetic Patient with Acute Kidney Injury: A Case Report
Uncontrolled diabetes mellitus is an immunosuppressive condition and leads to many secondary infections. There can be more than one micro-organism causing multiple organ system involvement in the same patient, in these s...