Detection of vancomycin intermediate and vancomycin resistance in clinical isolates of Staphylococcus aureus in Tertiary Care Hospital
Journal Title: International Journal of Medical and Health Research - Year 2019, Vol 5, Issue 6
Abstract
Introduction: Staphylococcus aureus is a major cause of variety of infections in health-care setting and community. In 1997 first case of infection by S. aureus with reduced susceptibility to vancomycin was documented in Japan. Soon several countries reported similar cases of infection due to this mutated pathogens. Objective: To isolate and identify Staphylococcus aureus and to detect VISA and VRSA from staphylococcus aureus isolates from clinical specimens. Material and Methods: A total of 400 Staphylococcal species isolated from the various clinical specimens were included in the study. Specimens were inoculated on Blood agar, Macconkey agar and Thioglycollate broth. The inoculated media were incubated aerobically at 37⁰C for 18-24 hours. Isolates were identified and confirmed as per laboratory steps of procedure (SOP) by the conventional methods. Vancomycin screen agar test, Epsilometer test (E-test) and Microbroth dilution were performed to detect VISA and VRSA strains. All the isolates were subjected to Antimicrobial susceptibility testing on Mueller-Hinton agar (MHA) by Kirby Bauer disc diffusion method. Results: Out of 400 cases most of the cases belong to 21-30 years of age (28%). In E test most (64.75%) were found to be sensitive to Vancomycin with MICs of 1 µg/ml and 141 isolates (35.25%) were 2µg/ml while in microbroth dilution mostly (70%) was found to be sensitive to Vancomycin with MICs of 1 µg/ml, followed by 2 µg/ml (30%). Overall Staphylococcus aureus were 100% sensitive to Vancomycin and Linezolid both in Kirby-Baur disc diffusion while Erythromycin were least sensitive (47.5%). Conclusion: Our results suggested that prevention of emergence and transmission of VISA/VRSA in each community is needed.
Authors and Affiliations
Maina Raigar, Aruna Vyas, Rajani Sharma, Bharti Malhotra
Keywords
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