Determination of Clozapine by Air Assisted Dispersive Liquid-Liquid Microextraction Based on Solidification of Organic Droplet Followed by HPLC in Human Serum

Abstract

Due to the challenges of maintaining serum levels in a safe therapeutic range (1–3 μM), Clozapine is one of the most promising medications for managing schizophrenia. Recently the barrier to the broader use of clozapine has been recognized to be the measurement of clozapine levels, which is however challenging due to the complexity of serum samples. The determination of clozapine is mainly conducted through a novel, simple, rapid and sensitive air assisted dispersive liquid-liquid microextraction based on solidification of organic droplet method that has been developed and validated by high-performance liquid chromatography with UV detection in human serum. In this method, due to the usage of an organic solvent with a low density and appropriate melting point, no microsyringe or fiber is required to support the organic microdrop. Furthermore, the extractant droplet can be collected easily by solidifying it at low temperature. 1-Undecanol was selected as extraction. The optimization of Parameters that influence extraction efficiency, i.e., volumes of extracting solvent, pH, and salt effect, were performed using a factor by factor approach. Enrichment factor for clozapine under optimal conditions was 128. The limit of detection was 1.34 ng/mL in serum sample. The proposed method was successfully applied to the determination of clozapine in serum samples of a person under clozapine therapy.

Authors and Affiliations

Amir Hosein Mohammad shafiee, Mohammad Reza Mohammad shafiee

Keywords

Related Articles

Green synthesis, characterization and antimicrobial activity of silver nanoparticles (AgNPs) using leaves and stems extract of some plants

Green synthesis of silver nanoparticles using the aqueous extract of Ferula gumosa, Ferula latisecta, Teucrium polium and Trachomitum venetum leaves and stems extract as the reducing and stabilizing agents. This synthesi...

Effect of partial substitution of raw gypsum with thermally treated phosphogypsum on the properties of Portland Pozzolanic Cement

For many years of production of phosphate fertilizer very large amounts of phosphogypsum (PG) occupied large area, causing chemical and radiological environmental. Using of PG in building materials represents a good meth...

Comparison of Elemental Contents of Some Edible Plant Leaves in Hong Local Government Area of Adamawa State, Nigeria

The elemental contents of Ficus sycomorus, Celtis interifolia, Balanites eagyptiaca, Vitex doniana and Haemtostaphis barteri leaves were investigated from Hong Local Government Area of Adamawa state. Atomic absorption sp...

Oil Spill Removal from Water by Absorption on Zinc-Doped Cobalt Ferrite Magnetic Nanoparticles

In the present study, Zn-doped cobalt ferrite (CoFe1.9Zn0.1O4) magnetic nanoparticles were successfully synthesized via sol-gel method. The prepared materials were characterized using X-ray powder diffraction (XRD), Four...

Preparation and biocompatibility evaluation of composite nanofibers with TiO2 nanoparticles

Medical device industry is growing at a very fast pace and a lot of activities have recorded over the past decade. Hence, it is very important the choice of blood compatible materials for use in the implants and medical...

Download PDF file
  • EP ID EP656164
  • DOI 10.33945/SAMI/AJCA.2020.2.1
  • Views 53
  • Downloads 0

How To Cite

Amir Hosein Mohammad shafiee, Mohammad Reza Mohammad shafiee (2020). Determination of Clozapine by Air Assisted Dispersive Liquid-Liquid Microextraction Based on Solidification of Organic Droplet Followed by HPLC in Human Serum. Advanced Journal of Chemistry-Section A (Theoretical, Engineering and Applied Chemistry), 3(2), 111-121. https://europub.co.uk/articles/-A-656164