DETERMINATION OF HYDROXYPROLINE IN COLLAGEN BIOMATERIALS FOR MEDICAL USE AND VALIDATION OF METHOD
Journal Title: Revista de Pielărie Încălţăminte - Year 2016, Vol 16, Issue 2
Abstract
The paper presents a method for determining hydroxyproline in collagen biomaterials for medical use produced in The Collagen Department of INCDTP – Division ICPI and its validation. The method has two stages: the first one consists in hydrolysis of proteins in the sample of protein product to the form of amino acids, among which hydroxyproline. The second stage refers to selective highlighting of hydroxyproline using a specific colour reaction and quantitative assessment against a reference standard with known concentration. The method was validated to establish performance parameter and to check compliance with the set goal.
Authors and Affiliations
Gabriela MACOVESCU, Ciprian CHELARU, Mădălina Georgiana ALBU KAYA, Luminiţa ALBU
Keywords
Related Articles
- EP ID EP533577
- DOI -
- Views 103
- Downloads 0
A LIMING PROCESS WITH SODIUM SILICATE COMPOUND AS A SWELLING AGENT
Low modulus sodium silicate was prepared with a polysilicon byproduct-silicon tetrachloride, and used as a swelling agent. Calcium chloride, hydrazine and protease were selected as auxiliaries for the swelling agent. Dos...
FOOTWEAR PROTECTION AGAINST FUNGI USING THYME ESSENTIAL OIL
Many natural antimicrobial agents have been identified over the last decades, such as essential oils, which have a proven effect against bacteria and fungi found on feet. This paper investigates antifungal activity of th...
THE EFFECT OF DIFFERENT SHOE OUTSOLE SHAPE ON PLANTAR LOADING DISTRIBUTION DURING RUNNING IN CHINESE MALE UNDERGRADUATE STUDENTS
The purpose of this study was to examine the effect of different shoe outsole shapes on plantar loading distribution during running. Three pairs of shoes with different outsole shapes were selected, which were Skechers G...
EFFECTS OF THE STRIDE LENGTH ON THE GAIT AND INTRA-LIMBS COORDINATION OF HEALTHY CHILDREN AGED 3 TO 6
Body motion adjusts while the stride lengths changes, but few studies report those influences on the gait mechanism and intra-limbs coordination of children; therefore, the purpose of our study was to evaluate the gait m...
COMPARISON OF SHOCK ABSORPTION PERFORMANCE OF BASKETBALL SHOE WITH DIFFERENT SOLE STRUCTURES
Basketball is a sport with high popularity, and it requires high on shoes. In violent experience, feet may be heavily impacted, which may cause damages to joints and nerves. Therefore, feet need the protection and shock...