Abstract

The objective of the present work was to quantify the concentration of non-esterified fatty acids, β- hydroxybutyrate, triacylglycerol and cholesterol during the negative energy balance, in Holstein cows. The study was carried out by sampling and analyzing 250 blood sera obtained from 50 Holstein cows. After delivery, the first sampling was performed at 7 d, the second at 15 d and the three remaining at 30, 45 and 60 d. Mean values for non-esterified fatty acids, β-hydroxybutyrate, triacylglycerol and cholesterol were determined. The resulting data set was analyzed by Gaussian distribution and descriptive statistics. Confidence intervals of 95% were established. The comparison between groups was carried out by Analysis of Variance and frequency histogram. The non-esterified fatty acids, β-hydroxybutyrate and triacylglycerol, 1 ABANICO VETERINARIO ISSN 2448-6132 Publisher Sergio Martinez Gonzalez abanicoacademico.mx/revistasabanico are consistent with the values reported internationally. The cholesterol concentration showed a slightly higher value than the references. The AGNE did not present differences between groups, the β- hydroxybutyrate registered its highest value at 15 d postpartum, and lowest at 60 d postpartum. Triacylglycerol and cholesterol showed their highest concentration at 60 d postpartum, and the lowest at 15 d postpartum. The calculated confidence intervals could be used at the herd level to detect alert situations when at least 5% of the cows in the sample fall outside the reference range.

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