Determination of Sildenafil Citrate in Powder Preparations Prepared from Revatio Tablets 20 mg for Infants with Persistent Pulmonary Hypertension of the Newborn
Journal Title: Scholars Academic Journal of Pharmacy - Year 2015, Vol 4, Issue 8
Abstract
The aim of the present study was to develop and validate a HPLC method for the assay of sildenafil citrate (SIL) in powder preparations prepared from Revatio Tablets 20 mg for infants with persistent pulmonary hypertension of the newborn. The good recovery of SIL from preparations was achieved using a methanol:0.1N HCl =1:1 solution as the extraction solvent. A chromatographic system comprising a YMC AM12S05-1506WT column, mobile phase of CH3CN:H2O:HClO4:NaClO4=400:600:1:5 (V/V/V/W), flow rate of 1 mL/min, and UV detector set at 290 nm showed good chromatographic separation for SIL and the internal standard (propyl 4-hydroxybenzoate). A regression analysis revealed that the method was linear over the standard curve range from 0.01 to 1 mg/tube. Inter-day precision values between the ranges were 4.3% or better. Accuracy values between the ranges were -0.9% - 7.0%, except for the value of 0.01 mg/tube. The accuracy value at 0.01 mg/tube was 16.6%, which indicated that the limit of quantitation of this method was 0.01 mg/tube. We herein demonstrated that this method is useful for determining SIL in a powder preparation prepared from Revatio tablets 20 mg
Authors and Affiliations
Tadakazu Tokumura, Miki Kawakami, Rika Kitada, Hideki Yamamoto, Takuro Kurita
Keywords
Related Articles
- EP ID EP143573
- DOI -
- Views 89
- Downloads 0
Hepatoprotective activity of herbal extract mixture (HEM) on ethanol induced hepatotoxicity in rats
Ayurveda an ancient Indian system of medicine claims to have remedy for disorders likes’ jaundice and liver cirrhosis, which is inadequate with present allopathy. The present study was conducted to find out the effective...
Deleterious effects of smoking in male patients in a tertiary care hospital
Abstract:This study explains the deleterious effects of cigarette smoking in patients of SSSMC&RI, Ammapettai. In this study we have collected a history of 75 patients in SSSMC&RI. This history has been collected based o...
Design, Synthesis and Pharmacological Evaluation of 2, 5 -Disubtituted-1, 3, 4-Oxadiazole Derivatives as Noval Antibacterial and Antifungal Agent
Abstract: The 1, 3, 4-Oxadiazole nucleus is associated with various pharmacological activities and are potential linker moieties. The Schiff base derivatives of 5-arylsubstituted-1, 3, 4-oxadiazole-2-amine were synthesiz...
Evaluation for Active Components and Nutrient Elements in Six Marketed Brands of Ispahani Tea Grown in Bangladesh
Abstract: Six marketed brands of Ispahani tea grown in Bangladesh viz; Best leaf, Best quality (BOP), Blender's choice, Best quality (PD), Zareen and Tea Bags were studied before expiry dates to screen out their active c...
Acute Toxicity in Mice and Effects of a Butanol Extract from the Leaves of Blighia Unijugata Bak. (Sapindaceae) on Electrocardiogram of Rabbits
Blighia unijugata is a plant used in traditional medicine to treat many pains and diseases such as fever, headaches, kidney pain and stiffness, dizziness and high blood pressure. No or few studies were carried out on its...