DETERMINATION OF THE RESIDUAL AMOUNT OF QUINOLONES IN POULTRY PRODUCTS BY MICROBIOLOGICAL METHOD
Journal Title: Бюлетень "Ветеринарна біотехнологія" - Year 2017, Vol 31, Issue
Abstract
One of priority areas of the state policy concerning public health nutrition is provision of foodstuff safety. Thus, features of their composition are taken into account, since, in addition to plastic material and biologically active substances, they can contain a lot of contaminants, including antimicrobials.The goal of the work was to conduct comparative analysis of the national and European microbiological screening methods for antibiotic residual detection in poultry meat, testing and validation of qualitative microbiological "NAT-screening" method of quinolones determination in poultry.Material and methods. The sensitivity and specificity limits were established by the microbiological method "NAT-screening" for the residues of quinolones determination. The study was carried out in 20 repetitions on simulated samples of poultry meat that are free from antibiotics and also in samples with introduced antibiotics solutions in concentrations of ½ MRL and MRL.Results of research and discussion. We found clearly defined growth inhibition zone of pure test culture YersiniaruckeriNCIM 13282 (АТСС 29473), which ranged from 16.01±0.01 mm to 20.03±0.03 mm. The result of the microbiological screening method "NAT-screening" study forquinolones residual amounts low detection (½ MRL) showed sensityvity to danofloxacin 100 μg/kg, difloxacin 150 μg/kg, enrofloxacin 50 μg/kg, flumequin 200 μg/kg oxolic acid 50 μg/kg in meat; denofloxacin - 200 μg/kg, diphloxacin - 950 μg/kg, enrofloxacin - 100 μg/kg, flumequin - 400 μg/kg, oxolic acid - 75 μg/kg - in the liver; denofloxacin - 200 μg/kg, diphloxacin - м300 μg/kg, enrofloxacin - 150 μg/kg, flumequin -500 μg/kg, oxolic acid - 75 μg/kg in the kidney, which corresponded MDR of European law and order № 695 from 06.08.2013.Conclusions and prospects for further research. Established that sensitivity, specificity and accuracy of the microbiological method "NAT-screening" for residual quinolones is 100%.
Authors and Affiliations
Ilona Azyrkina
Keywords
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