Determination of Triphenylmethane Dye in seafood by Liquid Chromatography and Tandem Mass Spectrometry
Journal Title: International Journal of Medical Science and Innovative Research (IJMSIR) - Year 2018, Vol 3, Issue 1
Abstract
Triphenylmethane dyes (TPMs) are synthetic dyes used for a wide range in industrial applications. Traditionally in aquaculture production TPMs is applied for antibacterial, anti-fungal, anti-parasitic properties due to the low cost. TPMs are mutagenic and carcinogenic to human beings; therefore US and EU have established a law of a zero tolerance of TMPs to be prohibited in agriculture, fisheries and other related industries. TPMS also exist some deficiencies, such as rapid metabolic property to form a reducing metabolite easily in the aquaculture products tissue. In analysis TPMS also have problems of complicated sample pretreatment and analysis instability of instrument. In this paper we develop a new simple method to improve the simplicity, efficiency and accuracy of analysis. The method was developed and validated to analyze the residues of seven triphenylmethane dyes and their metabolites (Malachite green, Leuco malachite green, Crystal violet, Leuco crystal violet, Methylene blue, Methyl Red, Azure B, Nile blue and Victoria Blue) in aquaculture products by using LC/MS/MS. The sample is prepared by a modified QuEChERS technique, which is Quick, Easy, Cheap, Effective, Rugged, and Safe. The optimal extraction and cleanup conditions are needed in experimental design. The validation parameters from the modified method need to meet the requirements of substances in aquaculture products, which establish by regulatory agencies. The results show that nine TPMs can be identified and quantified between 0.5 and 100 ng/mL of concentration and possessed the recovery between 100% and 97.1%(n=15). Under reproducible conditions, RSD had precision between 2.15% and 6.9%. Overall, the finding highlights the impact of simplicity, sensitivity and rapidity as well as the simultaneous determination of analytes in aquatic products
Authors and Affiliations
Su-O Chen
Keywords
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