Determination of Weak Du Antigen Among Rheusus Negative Blood Donors
Journal Title: Journal of Indus Medical College - Year 2018, Vol 1, Issue 1
Abstract
Introduction: Rh antigen is major blood group of Rh blood group system. The ‘’Weak D’’ antigen indicates the presence of aberrant expression of Rh -D protein on red cells. In vitro, Rh antigen does not react routinely by agglutination with potent monoclonal anti – D antisera, but it requires adding up of antiglobulin serum to detect the presence of antigen. Expression of weak D antigen is necessary in certain cases where risk of sensitization and allo-immunization is present. Objective: The main objective of this study is to detect the presence of ‘’Weak D’’ antigen in Rh-Negative blood donors. Methodology: This study was conducted at Department of Pathology, Indus Medical College, Tando Muhammad Khan during the period of 6 months (November 2017 to May 2018). A total of 2281 participants were included in this study. 3mL blood was collected from each participant for detection of ABO and Rh blood typing. Rh -Negative individuals were further evaluated for the presence of ‘’Weak-D antigen’’ with the addition of antihuman antiglobulin. Data was analyzed using SPSS 21.0. P – value of <0.05 was considered as statistically significant. Results: Out of 2281 participants, 2122 (93.02%) were Rh-Positive while 159 (6.97%) were Rh-Negative. Rh-Negative individuals were evaluated for the presence of weak D antigen. Out of 159 Rh-negative individuals, 8 (5.03%) were positive for weak D antigen with P – value of <0.001. Conclusion: Overall incidence of weak D antigen in our study was 0.3%. Detection of weak D antigen is not routinely performed in laboratories, but their presence may be important factor in certain cases where risk of sensitization and allo -immunization is high.
Authors and Affiliations
Shahzad Ali Jiskani, Umair Ali Soomro, Qandeel Soomro
Keywords
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Determination of Weak Du Antigen Among Rheusus Negative Blood Donors
Introduction: Rh antigen is major blood group of Rh blood group system. The ‘’Weak D’’ antigen indicates the presence of aberrant expression of Rh -D protein on red cells. In vitro, Rh antigen does not react routinely by...