Determining antifungal, anti-biofilm and anti-cancer activities of “1,3-di(thiophen-2-yl) prop-2-en-1-one” chalcone derivative
Journal Title: Cumhuriyet Medical Journal - Year 2017, Vol 39, Issue 2
Abstract
Objective: Our study aims to determine antifungal and anti-biofilm activity potential of five chalcone derivatives, which have been already reported in the literature, against Candida albicans and Candida tropicalis. In addition, it aimed to determine antioxidant property and anti-cancer activity of 1,3-di(thiophen-2-yl)prop-2-en-1-one chalcone derivative, whose antimicrobial activity was revealed within the study, in human breast cancer cell line (MCF-7) and human bone cancer cell line (MG63). Method: Chalcones used in the study were diluted two times in dimethyl sulfoxide (DMSO) and prepared at concentration between 1000-15 μg/mL. Antifungal and anti-biofilm activity of chalcones determined by using microdilution method. Radical scavenging activity was tested by employing 2, 2-diphenyl-1-picrylhydrazyl (DPPH). The cell viability was assessed using the cleavage of tetrazolium salts added to the culture medium. Results: In our study, MIC values of “1,3-di(thiophen-2-yl) prop-2-en-1-one”, which showed its antimicrobial activity on C. albicans and C. tropicalis, were found to be 0.06 mg/mL for both organisms, whereas its MBC values were found to be 0.12 mg/mL and 0.50 mg/mL respectively. MBIC activities were found to be 0.06 and 0.25 mg/mL respectively, whereas MBEC values were found to be 1 mg/mL. When MIC was applied to 48-hours biofilm formation, it was observed that the formation has decreased by 83% and 81%. IC50 value of “1,3-di(thiophen-2-yl) prop-2-en-1-one” was found to be 13,52 μg / mL. Regarding MCF-7 and MG63 cell line at 5 μg / mL concentration, 30% and 65% preserve its viability after 24 hours of incubation respectively. Conclusions: According to these results, it can be said that chalcone compound that include thiophen ring has shown an inhibiting effect on the multiplication and both on the formation of the biofilm of C. albicans and C. tropicalis strains. The results of the study revealed that chalcone compound may be valuable as an antifungal, anti-biofilm and anti-cancer agent.
Authors and Affiliations
Uğur Tutar, Cem Çelik, Hayreddin Gezegen, Ceylan Hepokur
Keywords
Related Articles
- EP ID EP238813
- DOI 10.7197/223.v39i29491.316366
- Views 88
- Downloads 0
Anorectal amelanocytic malignant melanoma: Case report
Primary anal malignant melanoma is a rare tumor of the anal region. Usually it was misdiagnosed as hemorrhoids or anal polyp so delayed diagnosis can lead complicated status of the disease.Clinical and histopathological...
MDM2 SNP309 polymorphism in Turkish population
Objective: Murine double minute 2 (MDM2) gene has a critical role on regulation of p53 tumor suppressor pathway. MDM2 is a major negative regulator of p53 function. MDM2 protein regulates p53 function through multiple in...
Assessment of the relationship between intraabdominal fat thickness and the aortomesenteric angle and distance using computed tomography
Objective: The aim of this study is to evaluate the effect of visceral fat thickness on the distance and angle between the superior mesenteric artery (SMA) and the aorta in the normal population. Method: The aortomesente...
Oncological and functional outcomes of 244 laparoscopic urological procedures: Cumhuriyet University experience
Objective: Presenting short-term oncological and functional outcomes of laparoscopic procedures performed in the past two years in the Urology Department of Cumhuriyet University Health Services Training and Research Hos...
A case with acute idiopathic blue finger
A case with acute idiopathic blue finger