DEVELOPMENT AND ADAPTATION TO LARGE-SCALE PRODUCTION IMMUNOENZYMATIC TEST SYSTEM INCREASED SENSITIVITY FOR EARLY DIAGNOSIS OF HIV INFECTION
Journal Title: Наукові доповіді НУБіП України - Year 2017, Vol 5, Issue 5
Abstract
More than 25 years the problem of the spread of the human immunodeficiency virus (HIV) continues to be relevant for the whole world. The problem of early diagnosis of the human immunodeficiency virus can be solved by introducing into the laboratory practice the 4th generation high-sensitivity diagnostic immunoenzyme test systems. The advantage of this combination analysis is the possibility of simultaneous detection in the blood serum of HIV-1 p24 antigen and HIV-specific focal antibodies (IgG, IgM, IgA), which allows detecting infection in the early stages of development. The fourth generation ELISA test system is constructed on the basis of purified recombinant proteins HIV-1, HIV-2 and monoclonal antibodies, which is adapted to large-scale production. Indicators of informativity of the enzyme immunoassay system "DIA-HIV-Ag / Ab" were analyzed, the analytical sensitivity according to the AVI standard was 2.5 pg / ml, and according to the NIBSC standard - 1 IU/ml.
Authors and Affiliations
T. Y. Trokhymchuk, L. A. Ganova, V. G. Serduk, M. Y. Spivak
Keywords
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