DEVELOPMENT AND ADAPTATION TO LARGE-SCALE PRODUCTION IMMUNOENZYMATIC TEST SYSTEM INCREASED SENSITIVITY FOR EARLY DIAGNOSIS OF HIV INFECTION
Journal Title: Наукові доповіді НУБіП України - Year 2017, Vol 5, Issue 5
Abstract
More than 25 years the problem of the spread of the human immunodeficiency virus (HIV) continues to be relevant for the whole world. The problem of early diagnosis of the human immunodeficiency virus can be solved by introducing into the laboratory practice the 4th generation high-sensitivity diagnostic immunoenzyme test systems. The advantage of this combination analysis is the possibility of simultaneous detection in the blood serum of HIV-1 p24 antigen and HIV-specific focal antibodies (IgG, IgM, IgA), which allows detecting infection in the early stages of development. The fourth generation ELISA test system is constructed on the basis of purified recombinant proteins HIV-1, HIV-2 and monoclonal antibodies, which is adapted to large-scale production. Indicators of informativity of the enzyme immunoassay system "DIA-HIV-Ag / Ab" were analyzed, the analytical sensitivity according to the AVI standard was 2.5 pg / ml, and according to the NIBSC standard - 1 IU/ml.
Authors and Affiliations
T. Y. Trokhymchuk, L. A. Ganova, V. G. Serduk, M. Y. Spivak
Keywords
Related Articles
- EP ID EP258155
- DOI -
- Views 92
- Downloads 0
THE EFFECT OF Nb-CONTAINING NANOCOMPOSITES BASED ON SAPONITE ON THE INDUCTION OF CHLOROPHYLL FLUORESCENCE IN LEAVES OF CORN
Today, nanotechnology opens up great opportunities in agriculture. Nanoparticles are used for presowing treatment of seeds of plants, resulting in its improved quality , increased resistance to phytopathogens and increas...
GEOCHEMISTRY OF HEAVY METALS IN SOILS OF THE ZONE OF INFLUENCE OF THE NONFERROUS METALLURGY ENTERPRISES
Objects of researches are soil adjournment under the influence of the non-ferrous metallurgy Industrial complex. The total maintenance and the maintenance of mobile forms of heavy metals defined by means of a method of n...
БІОЛОГІЧНА АКТИВНІСТЬ ҐРУНТУ ЕНЕРГЕТИЧНИХ КУЛЬТУР
Створення та експлуатація енергетичних культур не повинні призводити до виснаження ґрунтів. Дослідження цієї проблеми потребує вивчення ґрунтової мікробіоти як важливого компоненту ґрунту під цими рослинами. Об’єктами до...
HOW TO AVOID PUTANICS DURING THE QUALITY OF TEST RESULTS AND EVALUATION OF THE CONFORMITY OF TEST METHODS WHEN PERFORMING THE REQUIREMENTS OF ISO/IEC 17025: 2017 STANDARD IN ACCREDITED LABORATORY
This article objectively analyzes the problems that arise in the process of the activity of a modern accredited laboratory when implementing the requirements of ISO / IEC 17025: 2017 in terms of ensuring the quality of t...
THE TROPHIC COMMUNICATION OF THE COTTON BOLLWORM IN THE MODERN GROWING TECHNOLOGY OF SUNFLOWER AND CORN IN THE FOREST-STEPPE OF UKRAINE
The features of reproduction of cotton shoots of sunflower seeds and corn under modern agricultural systems in the forest-steppe of Ukraine are highlighted. The analysis of efficiency of application of modern monitoring...