DEVELOPMENT AND APPLICATION OF Pasteurella multocida SEROTYPE A:1 WHOLE CELL BASED COAGGLUTINATION TEST (CAT) FOR THE RAPID DETECTION OF FOWL CHOLERA CAUSING ORGANISMS
Journal Title: Journal of Experimental Biology and Agricultural Sciences - Year 2017, Vol 5, Issue 0
Abstract
Pasteurella multocida whole cell antigen based coagglutination test was optimized in the laboratory for the specific and sensitive identification of bacteria causing fowl cholera in a simple and cost effective manner. The test showed 100% specificity and sensitivity up to 3.4 ×106 formalinized bacterial cells. For testing stability, sensitized coagglutination test reagents were stored at 40C for 1-4 months was subjected to reactions with inactivated P. multocida type A:1, A:3 and A:4 and non Pasteurella cultures at the end of each month. The optimized test also detected P. multocida type A:1, A:4 in experimentally infected birds (n=10) indicating the potential application of the reagent to detect all possible somatic types of A capsular type which are etiological agents of Fowl cholera. P.multocida type B:2 experimentally infected mice (n=4) tissues also showed coagglutination with the reagent exemplify its wider applicability in field diagnosis.
Authors and Affiliations
Stanzin Zadon, Vinod Kumar Chaturvedi, Praveen Kumar Gupta, Varsha Paladan, Perumal Nanda Kumar
Keywords
Related Articles
- EP ID EP427745
- DOI 10.18006/2017.5(6).767.773
- Views 64
- Downloads 0
Effect of nitrogen and sulfur on the quality of the cotton fiber under mediterranean conditions
Agronomic practices significantly influence the productivity and quality of cotton plant. Present study was undertaken to evaluate the effect of nitrogen and sulfur fertilizer application on the fiber quality of cotton,...
Effect of papaya and storage time on the quality of the newly developed papaya crackers
Crispy nutritious papaya crackers were developed from locally available papaya and whole wheat flour by baking in oven at 130oC for 35 min. Crackers were packed in high density polyethylene pouches and stored under ambie...
Effect of non-catalytic infrared ray on the grain moth Sitotroga cerealella (lepidoptera: gelechiidae)
This study was conducted under laboratory conditions to find out the effect of the non-catalytic infrared on the mortality rates and development of various stages of the grain moth Sitotroga cerealella. Result of the stu...
PHYLOGENETIC ANALYSIS OF Arcobacter butzleri AND Arcobacter skirrowii ISOLATES AND THEIR DETECTION FROM CONTAMINATED VEGETABLES BY MULTIPLEX PCR
Arcobacter is an identified emerging food-borne bacterial pathogen. In the current study, 204 different vegetable samples were collected from retail shops or local vendors from northern part (Uttar Pradesh) of India and...
EFFECT OF PROBIOTIC AND PRILLED FAT ON LACTATION PERFORMANCE OF CROSS BRED COW DURING TRANSITION PERIOD
In present study, combined effects of prilled fat and yeast culture (YC) mixture supplemented diet on 12 early lactating crossbred cows were evaluated. Cows were divided into two groups as control group (CON group) and s...