DEVELOPMENT AND CHARACTERIZATION OF CHITOSAN-ALGINATE SCAFFOLD AS POTENTIAL FOR WOUND HEALING
Journal Title: WORLD JOURNAL PHARMACY AND PHARMACEUTICAL SCIENCE - Year 2019, Vol 8, Issue 11
Abstract
The present work aims at the development and characterization of chitosan (Ch) and alginate (Alg) Scaffold (Scf) and demonstrates the effect of depolymerization on the Chitosan-based scaffold compared with the non-depolymerized form. Thus, medium molecular weight Ch was purified and depolymerized, mixed with Alg and then lyophilized to obtain functional Scf. Morphological analyses by Scanning Electron Microscope (SEM) were performed to estimate porosity and pore size; Physicochemical essays by Fourier-transform Infrared Spectroscopy (FTIR) and swelling behaviour were made to evaluate the available functional groups of Scf and water uptake, respectively. The SEM showed a better porosity in the depolymerized form, the pore sizes evaluation displayed a range from 24 up to 298 μm, with a predominance at 160 μm, the images also shows the interconnectivity within the pores. The FTIR demonstrates that the depolymerization changes the spectrum of Ch and that there is an interaction between the two polymers, it also shows the presence of functional groups, important for cell attachment. The swelling behaviour demonstrated a higher water uptake capacity (4500 to 5000%) at acidic pH in comparison with the slightly alkaline pH of 7,2, 1000 to 2500%, in the Scf non-depolymerized and depolymerized, respectively. In conclusion, the proper porosity, pore size and swelling behaviour of dpCh-Alg Scf, proved its potential for further in vitro and in vivo studies, not only as wound healing in skin tissue, but could also be a potential matrix for the culture of many different kinds of eukaryotic cells, which could be a good tool in tissue engineering related to regeneration.
Authors and Affiliations
Rosa Valéria Amorim
Keywords
Related Articles
- EP ID EP669812
- DOI -
- Views 135
- Downloads 0
APPLICATIONS OF LIPOSOMES IN OPHTHALMIC DRUG DELIVERY SYSTEM
Drug delivery research to the eye has gathered momentum over the past few years in an attempt to overcome the eyes natural barriers to drugs. Since the eye can be affected by a multitude of diseases such as infections an...
ROLE OF POLYOLS AND SURFACTANTS IN LIQUID PROTEIN FORMULATIONS
in aqueous protein formulations, increase conformational stability of proteins by size exclusion mechanism, increasing thermodynamic stability of proteins and decreasing aqueous solubility. Polyols prevent protein aggreg...
THE ECONOMIC OUTCOMES OF THE MEDICATION SAFETY OFFICER IN THE CARDIOLOGY SPECIALTY
Introduction: no studies highlighted the economic outcomes of the medication safety officer position in cardiology specialty in the Middle East hospitals. Objective: to determine the economic outcomes of the medication s...
ANTIOXIDATIVE POTENTIALS OF COOKED BAMBARA GROUNDNUT BASED DIET (VIGNA SUBTERRANEAN) ON LIPID PEROXIDATION STATUS IN PRETREATED ALLOXAN INDUCED DIABETIC RATS
The present study examines the antioxidant potentials of Cooked Bambara groundnut (Vigna subterranean) based diet in pretreated alloxan induced diabetic rats. Thirty (30) male albino rats (Rattus novergicus) were ramdoml...
ADJUNCTIVE USE OF ESOMEPRAZOLE IN THE TREATMENT OF PRE-ECLAMPSIA: A CASE REPORT AND LITERATURE REVIEW
Faced with the absence of suitable assays and/or kits for predictive biomarkers, medical practitioners in poor resource settings may be emasculated by the unrelenting upwards prevalence of and uncertain forecast associat...