DEVELOPMENT AND VALIDATION FOR THE SIMULTANEOUS ESTIMATION OF DACLATASVIR AND SOFOSBUVIR IN DRUG PRODUCT BY RP-HPLC
Journal Title: Indo American Journal of Pharmaceutical Research - Year 2018, Vol 8, Issue
Abstract
New Analytical method was developed for the estimation of Daclatasvir and Sofosbuvir in drug product by liquid chromatography. The chromatographic separation was achieved on C18 column (Phenyl XDB 250*4.6mm) at ambient temperature .The separation achieved employing a mobile phase consists of 0.1%v/v Trifluoro acetic acid in water : Acetonitrile(500:500). The flow rate was 1.0ml/ minute and ultra violet detector at 275nm. The average retention time for Daclatasvir and Sofosbuvir found to be 2.805 min and 3.743 min. the proposed method was validated for selectivity, precision, linearity and accuracy. All validation parameters were within the acceptable range. The assay methods were found to be linear from 12.0 – 36.0μg/ml for Daclatasvir and 80.0 – 240.0 μg/ml of Sofosbuvir.
Authors and Affiliations
Keywords
Related Articles
- EP ID EP490243
- DOI -
- Views 108
- Downloads 0
COMPARATIVE STUDY AND ANALYSIS OF RELEASE KINETICS OF CORIANDER FORMULATIONS
Acne is an inflammatory disease of sebaceous follicles of skin. The present study was conducted to formulate and evaluate the topical anti acne formulation of coriander oil and coriander aqueous extract with and without...
ANAESTHESIA-THE PRIME IMPORTANCE OF SURGERY & FASTING REQUIREMENTS REVIEW
Abstract Anesthesia is temporarily induced loss of sensation. It includes -analgesia(loss of pain), amnesia(loss of memory),unconsciousness (reversible loss of consciousness that is coma not sleep), paralysis(muscle rela...
HYPROMELLOSE AND CARBOMER INDUCE BIOADHESION OF ACYCLOVIR TABLET TO VAGINAL MUCOSA
The present research indicates fabrication of an “economic” controlled release bioadhesive vaginal tablets containing acyclovir using only two polymers; Carbopol 934P (as bioadhesive agent) and HPMC K15 (as rate retardan...
MONITORING OF ADVERSE DRUG REACTIONS (ADR) IN CHRONIC MYELOID LEUKEMIA (CML) PATIENTS TREATED WITH IMATINIB AT A TERTIARY CARE HOSPITAL
Imatinib is a tyrosine kinase inhibitor and potently inhibits protein tyrosine kinases which include BCR-ABL which is constitutively active in CML. It targets the ATP binding site of receptors. The present investigation...
DEVELOPMENT AND EVALUATION OF CARBOPOL BASED SUATAINED RELEASED MATRIX TABLETS OF TRAMADOL HYDROCHLORIDE
Abstract In the present investigation an attempt has been made to increase therapeutics efficacy, reduce frequency of administration and improve patient compliance by developing Sustained release matrix tablets of tramad...