Development and validation of a capillary electrophoresis method for determination of oxcarbazepine and zonisamide in pharmaceuticals
Journal Title: Journal of Pre-Clinical and Clinical Research - Year 2008, Vol 2, Issue 2
Abstract
A simple, rapid capillary electrophoretic (CE) method has been evaluated and validated for determination of two newer antiepileptic drugs: oxcarbazepine and zonisamide, as pure substances and in tablet form. The method was developed by employing fused silica capillary with an effective length of 80 cm and internal diameter of 75 μm. 0.067 M phosphate buffer with pH 7.10 was used for oxcarbazepine analysis and 0.1 M acetate buffer with pH 4.58 was used for zonisamide determination. The recommended applied voltage, capillary temperature and injection time were 30 kV, 30°C and 10 s, respectively. Detection was followed by direct absorptiometric measurements at 210 nm (oxcarbazepine) or 240 nm (zonisamide). Developed methods were linear in the range of 5–100 μg/mL, and had good correlation coefficients (r=0.9999 for oxcarbazepine and r=0.9995 for zonisamide). Intra- and inter-day precision, calculated as relative standard deviation (RSD), were better than 2.0 %. The proposed CE methods were successfully applied to the assay of oxcarbazepine and zonisamide in pharmaceutical formulations.
Authors and Affiliations
Beata Paw, Katarzyna Woś
Keywords
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