Development and Validation of a High-performance Liquid Chromatography–Diode-array Detection Method for the Determination of Eight Phenolic Constituents in Extracts of Different Wine Species
Journal Title: Turkish Journal of Pharmaceutical Sciences - Year 2018, Vol 15, Issue 2
Abstract
Objectives: A new HPLC method was developed and validated for the determination of some phenolic compounds; gallic acid, chlorogenic acid, epigallocatechin, caffeic acid, vanillin, p-coumaric acid, rutin, and quercetin in some local wine and fruit wine samples. Materials and Methods: Analyses were performed on a Zorbax Eclipse C18 column (4.6 x 150 mm, 3.5-µm particle size) using a gradient system. Mobile phase A was a 10-mM phosphoric acid solution and mobile phase B was methanol using a flow rate of 1 mL/min. Phenolic components were monitored using a DAD at three different wavelengths. Results: The developed and validated method was generally linear between the 1-100 ppm concentration range. Recovery values were obtained in the range of 95-105% and repetitive. The method was successfully applied to investigate the phenolic profiles of different wine samples. Conclusion: As a result of the study, an accurate, sensitive and reliable HPLC-DAD method was developed. The method was successfully used to determine the concentrations of antioxidant phenolic constituents from some local wine extracts.
Authors and Affiliations
Ebru TÜRKÖZ ACAR, Mehmet Engin CELEP, Mohammad CHAREHSAZ, Gülşah Selin AKYÜZ, Erdem YEŞİLADA
Keywords
Related Articles
- EP ID EP382435
- DOI 10.4274/tjps.54154
- Views 122
- Downloads 0
In Vivo Effects of Naringenin and Lead on Rat Erythrocyte Carbonic Anhydrase Enzyme
Objectives: Carbonic anhydrase (CA) enzyme catalyses the reversible reactions of CO2 with water and takes part in metabolically important events such as systemic acid-base regulation and respiration. In this study, in vi...
Encapsulation of Flurbiprofen by Chitosan Using a Spray-Drying Method with In Vitro Drug Releasing and Molecular Docking
Objectives: This study aimed to prepare chitosan-flurbiprofen micro-nano spheres as environmentally friendly for drug releasing by spray-drying method without any cross-linking agent. It was also aimed to reveal the favo...
Development of Andrographolide-Carboxymethyl Chitosan Nanoparticles: Characterization, in vitro Release and in vivo Antimalarial Activity Study
Objectives: The purpose of this study was to investigate the effect of andrographolide-carboxymethyl chitosan nanoparticles formation on the physical characteristics, in vitro release profile and in vivo antimalarial act...
Assessment of Genotoxic Effects of Pendimethalin in Chinese Hamster Over Cells by the Single Cell Gel Electrophoresis (Comet) Assay
Objectives: Pendimethalin (N-(1-ethylpropyl)-3,4-dimethyl-2,6-dinitrobenzeneamine) is a dinitroaniline herbicide compound which selectively controls weeds. It is a cell division and growth inhibitor. It descends plants...
Melatonin in Edible and Non-Edible Plants
The concept of melatonin has become more important recently both in plants and in human who utilize plants for nutritional and health purposes. Melatonin, synthesized from L-tryptophan by enzyms, protects plants against...