Development and Validation of a High-performance Liquid Chromatography–Diode-array Detection Method for the Determination of Eight Phenolic Constituents in Extracts of Different Wine Species
Journal Title: Turkish Journal of Pharmaceutical Sciences - Year 2018, Vol 15, Issue 2
Abstract
Objectives: A new HPLC method was developed and validated for the determination of some phenolic compounds; gallic acid, chlorogenic acid, epigallocatechin, caffeic acid, vanillin, p-coumaric acid, rutin, and quercetin in some local wine and fruit wine samples. Materials and Methods: Analyses were performed on a Zorbax Eclipse C18 column (4.6 x 150 mm, 3.5-µm particle size) using a gradient system. Mobile phase A was a 10-mM phosphoric acid solution and mobile phase B was methanol using a flow rate of 1 mL/min. Phenolic components were monitored using a DAD at three different wavelengths. Results: The developed and validated method was generally linear between the 1-100 ppm concentration range. Recovery values were obtained in the range of 95-105% and repetitive. The method was successfully applied to investigate the phenolic profiles of different wine samples. Conclusion: As a result of the study, an accurate, sensitive and reliable HPLC-DAD method was developed. The method was successfully used to determine the concentrations of antioxidant phenolic constituents from some local wine extracts.
Authors and Affiliations
Ebru TÜRKÖZ ACAR, Mehmet Engin CELEP, Mohammad CHAREHSAZ, Gülşah Selin AKYÜZ, Erdem YEŞİLADA
Keywords
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- EP ID EP382435
- DOI 10.4274/tjps.54154
- Views 132
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