Development and Validation of a HPLC-UV Method with Pre-column Derivatization for Determination of Cinnabar in Jufang Zhibao Pills
Journal Title: Indian Journal of Pharmaceutical and Biological Research - Year 2017, Vol 5, Issue 2
Abstract
In this work, a reliable and accurate high-performance liquid chromatography method with pre-column derivatization was established and validated for determination of cinnabar in Jufang Zhibao pills. Scanning electron microscope (SEM) image was used to identify the types of cinnabar crude drug in Jufang Zhibao pills. The chromatography separation was performed on a Welch XB-C18 column (250 mm × 4.6 mm, 5 μm). The mobile phase consists of water spiked with 0.022 mmol/L sodium diethyldithiocarbamate (A, pH adjusted to 8–9 by ammonia water) and methanol (B, 80:20, v/v) at flow rate of 1.0 ml/min with the detected wavelength was 272 nm. The oven temperature was set at 35°C. The calibration for cinnabar content has good linearity (R2 =0.9999) over the range of 2.43–300 μg/ml and the average recovery was less then 1.90%. The limits of detection and quantification were 0.1127 μg and 0.2065 μg/ml. The results indicated that the proposed method has advantages of high accuracy, good repeatability and stability and can be successfully used for determination of cinnabar in Jufang Zhibao pills. It provides a basis for drug manufacture quality control and proves the feasibility of the pre-column derivatization method during the determination of cinnabar in Jufang Zhibao pills.
Authors and Affiliations
Xin Zhang| Department of Traditional Chinese Medicine, Xi’an Institute for Food and Drug Control, Xi’an 710054, China, Jia Yao| Department of Traditional Chinese Medicine, Xi’an Institute for Food and Drug Control, Xi’an 710054, China, Ying Ren| Department of Traditional Chinese Medicine, Xi’an Institute for Food and Drug Control, Xi’an 710054, China, Yuan Li| Department of Traditional Chinese Medicine, Xi’an Institute for Food and Drug Control, Xi’an 710054, China, Zhimin Xie| Department of Traditional Chinese Medicine, Xi’an Institute for Food and Drug Control, Xi’an 710054, China
Keywords
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