Development and validation of a RP- HPLC method for the quantitation studies of bormadiolone in Ratitox F
Journal Title: Medicamentul Veterinar / Veterinary Drug - Year 2011, Vol 5, Issue 2
Abstract
An isocratic high-performance liquid chromatography (HPLC) procedure was developed for quantitative determination of bromadiolone (hydroxycoumarins), in RATITOX F products–rodenticide. HPLC separation was carried out by reversed phase chromatography ODS 2 HYPERSIL C18 (250 mm x 4.6 mm i.e.; 5 µm particle size), held at 25C. The mobile phase consisted of methanol/ 0.1% aqueous solution phosphoric acid (90/ 10 v/ v), run at flow rate of 1 mL/ min and with UV detection at 265 nm. Method validation investigated parameters such as linearity (r2=0.9999), range, precision, accuracy, specificity, limit of detection and limit of quantification. The described method can be successfully applied for the analysis of RATITOX F–rodenticide.
Authors and Affiliations
Elena Oltean
Keywords
Related Articles
- EP ID EP113896
- DOI -
- Views 91
- Downloads 0
Effect of fenbendazole treatment in an ovine Dictiocaulosis episode in Timis County
Dicticocytosis of sheep is known as verminous bronchopneumonia,cosmopolitan geohelminthosis produced by Dictyocaulus filaria, which parasites in the trachea and the bifurcation of the large bronchi in sheep and goats. Cl...
Development and validation of a RP–HPLC method for the quantization studies of albendazole suspensions dosage forms of Rombendazol
An isocratic high–performance liquid chromatography (HPLC) procedure was developed for quantitative determination of albendazole in suspensions dosage forms of ROMBENDAZOL. HPLC separation was carried out by reversed pha...
Report concerning results of proficiency testing laboratory on assay of tobramycine and nystatin by microbiological method
The present study describes the results obtained by the Microbiological Control Laboratory from Institute for Control of Biological Products and Veterinary Medicines after participating in the proficiency testing scheme...
European perspectives in the field of medicinal products
European legislation governing veterinary medicinal product is under review by the European Commission. The new legislation will cover areas for research, development and authorization of new medicinal products and deter...
Elemente de terapie alternativă și complementară în medicina veterinară
No abstract