Development and validation of a RP- HPLC method for the quantitation studies of bormadiolone in Ratitox F
Journal Title: Medicamentul Veterinar / Veterinary Drug - Year 2011, Vol 5, Issue 2
Abstract
An isocratic high-performance liquid chromatography (HPLC) procedure was developed for quantitative determination of bromadiolone (hydroxycoumarins), in RATITOX F products–rodenticide. HPLC separation was carried out by reversed phase chromatography ODS 2 HYPERSIL C18 (250 mm x 4.6 mm i.e.; 5 µm particle size), held at 25C. The mobile phase consisted of methanol/ 0.1% aqueous solution phosphoric acid (90/ 10 v/ v), run at flow rate of 1 mL/ min and with UV detection at 265 nm. Method validation investigated parameters such as linearity (r2=0.9999), range, precision, accuracy, specificity, limit of detection and limit of quantification. The described method can be successfully applied for the analysis of RATITOX F–rodenticide.
Authors and Affiliations
Elena Oltean
Keywords
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