Development and validation of a RP–HPLC method for the quantitation studies of fipronil in Parakill suspensions
Journal Title: Medicamentul Veterinar / Veterinary Drug - Year 2011, Vol 5, Issue 2
Abstract
An isocratic high–performance liquid chromatography (HPLC) procedure was developed for quantitative determination of fipronil in suspensions dosage forms of PARAKILL. HPLC separation was carried out by reversed phase chromatography Betasil C18 (250 mm x 4.6 mm i.d.; 5 µm particle size), held at 25C. The mobile phase consisted of acetonitrile/ distillated water (60/ 40 v/ v), run at flow rate of 1 mL/ min and with UV detection at 220 nm. Method validation investigated parameters such as linearity (r2=0.9999), range, precision, accuracy, specificity, limit of detection and limit of quantification. The described method can be successfully applied for the analysis of PARAKILL suspensions.
Authors and Affiliations
Elena Oltean
Keywords
Related Articles
- EP ID EP119332
- DOI -
- Views 116
- Downloads 0
S-a ales continuitatea
Este prezentată noua componență a Consiliului director a Asociatiei Nationale a Fabricantilor de Produse de Uz Veterinar votată la ședința din 15 noiembrie 2011 a adunării generale a Asociației.
Demonstrating the antinociceptive action of renin-angiotensin system modulators in mice
Research done in the present study belongs to a wider area of experimental determinations on nociception models, when using laboratory animals. Their aim is to determine the ED50 value (Efficient dose 50) in conditions o...
Aniversarea a 85 de ani de viață a Prof. Dr. Dr.H.C. Iustin Cosoroabă
Celebrating 85 years of Prof. Dr. Dr.hc Justin Cosoroabă
Despre tarantule – posibile utilizări în medicină
In recent years, tarantulas have become increasingly popular in terrariums because they are easy to maintain, do not require much space and because of their beauty. Venom from spiders, snakes, fish, snails and scorpions...
Terapeutic eficacity testing of Rombendazol 10% (Romvac) and Fasinex (Novartis) in natural infestation with Fasciola hepatica at sheep from Bilbor, Harghita County
The study was carried out between January -February 2008, on 30 sheep Tigaie and Turcana breeds from Bilbor, Harghita County. Animals were distributed randomly into three groups (n = 10): group A1 (treated with albendazo...