DEVELOPMENT AND VALIDATION OF A SIMPLE HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY UV METHOD FOR THE DETERMINATION OF VINORELBINE - A CHEMOTHERAPEUTIC DRUG IN SPIKED HUMAN PLASMA
Journal Title: Asian Journal of Pharamceutical and Clinical Research - Year 2019, Vol 12, Issue 1
Abstract
Objective: Vinorelbine (VNRB) is a chemotherapeutic drug used to treat non-small cell lung cancer and breast cancer. Literature survey reveals that there are no reverse-phase high-performance liquid chromatography (RP-HPLC) methods reported for the estimation of VNRB in spiked human plasma. Hence, the present work aimed to develop a simple and efficient RP-HPLC method for the estimation of VNRB in human plasma. Methods: Specimen preparation for the measurement of VNRB was performed through liquid-liquid extraction using methanol as extracting solvent and reconstructed with mobile phase. Paclitaxel (PCTX) was used as internal standard. HPLC method was optimized and validated as per the US FDA bioanalytical guidelines. VNRB and internal standard were separated on Kromasil® C18 (250×4.6 mm; id 5 μ) using acetate buffer (pH=5.9) and methanol in the ratio of 85:15 (v/v) at 1 ml/min flow rate. Eluted compounds were recorded using UV detector at 235 nm. Results: The retention time of PCTX and internal standard was found to be 4.3 and 9.0 min, respectively. The analytical measuring ranges were found to be 5–750 ng/ml (r2>0.9998). The method was found to be simple, accurate, precise, and stable and there is no interference of plasma matric components. Conclusion: The described HPLC method allows for the measurement of total and free PCTX in both plasma and cord blood and can utilize for the estimation of drug in pharmacokinetic studies.
Authors and Affiliations
Useni Reddy Mallu, Venkateswara Rao Anna, Bikshal Babu Kasimala
DESIGN AND EVALUATION OF TORSEMIDE CONTROLLED RELEASE MATRIX TABLETS
 Objective: The controlled release Torsemide matrix tablets were prepared using different natural polymers in various proportions as releaseretarding agents to control the torsemide release and to improve the patient co...
AUTOMATIC FEATURE EXTRACTION FOR BREAST DENSITY SEGMENTATION AND CLASSIFICATION
Objective: Cancer is the uncontrollable multiplication of cells in human body. The expansion of cancerous cells in the breast area of the women is identified as breast cancer. It is mostly identified among women aged abo...
PALATAL RUGOSCOPY AS A METHOD OF SEX DETERMINATION IN FORENSIC SCIENCE
 Objective: The role of forensic science in establishing the personal identity is based on DNA analysis, finger print and dental analysis. When theforensic remains are decomposed making all these sources, unavailable pa...
COMPARATIVE ASSESSMENT OF THERAPEUTIC POTENTIAL OF VACHADI AND HINGWADI GHTRITA IN PATIENTS OF DEPRESSION
 Objective: Present exploratory clinical study dealt with comparative assessment of therapeutic effect of medicated ghee formulations i.e. Vachadi [VG] and Hingwadi Ghrita [HG] in patients of mild to moderate depression...
ISOLATION OF SECONDARY METABOLITE COMPOUNDS AND ANTIBACTERIAL ACTIVITIES TESTS FROM HEXANE EXTRACT OF STEM BARK MELOCHIA UMBELLATA (HOUTT) STAPF VAR. DEGRABRATA K
Objectives: This research aims to determine the content of secondary metabolite compounds and antibacterial activity of stem bark extract Melochia umbellata (Houtt) Stapf var. degrabrata.Methods: M. umbellata stem bark w...