DEVELOPMENT AND VALIDATION OF A UPLC METHOD FOR SIMULTANEOUS QUANTIFICATION OF DICLOFENAC POTASSIUM AND CHLORZOXAZONE IN THEIR BINARY MIXTURES AND PHARMACEUTICAL FORMULATION
Journal Title: WORLD JOURNAL PHARMACY AND PHARMACEUTICAL SCIENCE - Year 2019, Vol 8, Issue 9
Abstract
A simple, precise, accurate and rapid isocratic reverse phase Ultra high performance liquid chromatographic method was developed for simultaneous determination of diclofenac potassium and chlorzoxazone in binary mixture and capsules dosage form. The chromatographic separation was performed on an A zorbax eclipse plus C18 column (4.6mm X 100mm, 3.5μm) using a mobile phase composed of methanol: water acidified with phosphoric acid buffer (80:19:1 by volume) running on a flow rate of 1 mL/min and the effluent was monitored at 275 nm. The proposed method was validated for linearity, accuracy, precision, specificity, LOD and LOQ. The calibration was linear over the range of 10-80 ng/mL for diclofenac potassium and 50-400 ng/mL for chlorzoxazone. The retention times were found as 1.407 and 2.44 min for diclofenac potassium and chlorzoxazone; respectively. The proposed method was validated as per ICH guidelines and successfully applied for the quantitative routine determination of DFP and CLZ in their binary mixture and pharmaceutical dosage forms.
Authors and Affiliations
Bilal Amer Abdel Aal
SYNTHESIS, IDENTIFICATION AND ANTIBACTERIAL EVALUATION OF SOME 1,3,4- OXADIAZOLES DERIVATIVE ON 1,8-NAPHTHYRIDINE RING
Several 1,8-naphthyridine derivative were prepared using Vilsmeier-Haack synthization. 2-chloro-3-formyl-1,8-naphthyridine was synthesized by reacting N-(pyridin-2-yl) acetamide with POCl3 in dimethylformamide. 1,8-napht...
RED CELL DISTRIBUTION WIDTH: A USEFUL MARKER OF DISEASE ACTIVITY IN INFLAMMATORY BOWEL DISEASE
Background and Objective: Studies concerning red cell distribution width (RDW) for use in the assessment of inflammatory bowel disease (IBD) activity are limited. We investigated whether RDW is a useful clinical marker o...
CHEMO-SUPPRESSIVE ACTIVITIES OF CLOTRIMAZOLE AND GABAPENTIN IN A MURINE MALARIAL MODEL
AIM: To assess the chemo-suppressive activities of clotrimazole and gabapentin in a murine malarial model by four-day suppressive test. STUDY DURATION: This study was done between June and December, 2017. METHOD: Clotri...
IMPLEMENTATION OF A COMMUNITY PILOT CARDIOVASCULAR HEALTH PROGRAM
Mexico is currently suffering from serious problems of obesity based on data from the national survey of health - ENSANUT – 2016[1], shows that 70% of adults aged 30-60 suffer from overweight or obesity, seven in 10 adul...
INFLUENCE OF REGULATORY MEASURES AND MARKET POLICY FOR REIMBURSED MEDICINAL PRODUCTS ON THE VALUE, PAID WITH PUBLIC FUNDS IN BULGARIA IN 2015
In Bulgaria large number of medicines yearly are included and excluded from Positive Drug List (PDL). It leads to different changes in the value per DDD paid with public funds (reference value) and utilization of medicin...