DEVELOPMENT AND VALIDATION OF ANALYTICAL METHOD FOR DETERMINATION OF ESOMEPRAZOLE SODIUM BY HPLC
Journal Title: International Research Journal of Pharmacy (IRJP) - Year 2016, Vol 7, Issue 5
Abstract
A very simple, accurate, specific, precise and rapid reverse phase high performance liquid chromatography technique has been built up as well as validated to determine the Esomeprazole Sodium. The method is applicable for the quantitative analysis of the drug substance. Chromatographic separation was achieved on a Supelco 250-mm, 4.6-mm, and 5-μm C-18 analytical column with a mixture of Buffer and Acetonitrile at a volume ratio of 55:45 (v/v) as mobile phase at a flow rate of 1.0 mL /min. The molecule eluted within a short runtime (within 7.0 min). The eluted compound was monitored at a detector wavelength of 302 nm and the column oven temperature was maintained at 25 °C. The developed method was validated according to ICH guidelines. The high recovery and low relative standard deviation confirm the suitability of the method for determination of Esomeprazole Sodium. The repeatability and intermediate precision, expressed by the % RSD, were less than 2%. Accuracy (% recovery: 98.00-102.00%) was found to satisfactory. The method was validated by determining its accuracy, precision, system suitability, linearity and robustness. Validation studies reveal that the method is simple, specific, rapid, reproducible, precise and accurate, which is useful for the routine determination of Esomeprazole Sodium.
Authors and Affiliations
Farhana Lipi , Md. Islam , Shahida Parvin
Keywords
Related Articles
- EP ID EP106981
- DOI 10.7897/2230-8407.07543
- Views 137
- Downloads 0
DAPAGLIFLOZIN: SELECTIVE SODIUM-GLUCOSE CO-TRANSPORTER-2 INHIBITOR IN TYPE 2 DIABETES
Dapagliflozin is a promising new drug that targets the so far untapped renal glucose reabsorption. By inhibiting sodium-glucose co-transporter-2 (SGLT2) which is mainly localized in the S1 segment of the proximal tubule,...
THE IMPACT OF EDUCATIONAL INTERVENTION STRATEGIES IN REDUCING MEDICATION ERRORS IN A TARTAIRY CARE TEACHING HOSPITALTHE IMPACT OF EDUCATIONAL INTERVENTION STRATEGIES IN REDUCING MEDICATION ERRORS IN A TARTAIRY CARE TEACHING HOSPITAL
Medication error can increase the cost, prolong hospital stay and increase the risk of death almost two fold. Several studies have already demonstrated that pharmacist can play major role in detection and prevention of m...
PHYTOCHEMICAL AND PHARMACOLOGICAL PROFILE OF LAGENARIA SICERARIA: AN OVERVIEW
Cucurbitaceae family is major source of medicinal agents since ancient time. Various plants parts including fruits of this family have been established for their pharmacological potential. Lagenaria siceraria (Molina) st...
BUCCAL DRUG DELIVERY SYSTEM: THE CURRENT INTEREST
This review highlights the several advantages of buccal drug delivery system (BDDS) over the conventional and systemic formulation majorly. It helps to enhance bioavailability through bypassing the first pass metabolism....
SIMULTANEOUS ESTIMATION AND VALIDATION OF PARACETAMOL AND DOMPERIDONE IN BULK AND TABLET DOSAGE FORM BY USING DIFFERENT SPECTOPHOTOETRIC METHOD
Three simple, accurate and precise spectrophotometric methods have been developed for simultaneous determination of Paracetamol and Domperidone in a binary mixture. In Method I absorbance were measured at 258 nm an...