DEVELOPMENT AND VALIDATION OF DUAL WAVELENGTH SPECTROPHOTOMETRIC METHOD FOR SIMULTANEOUS ESTIMATION OF CEFIXIME TRIHYDRATE AND OFLOXACIN IN TABLET DOSAGE FORM
Journal Title: International Research Journal of Pharmacy (IRJP) - Year 2011, Vol 2, Issue 7
Abstract
The present manuscript describe simple, sensitive, rapid, accurate, precise and economic dual wavelength spectrophotometric method was developed for the simultaneous determination of cefixime trihydrate (CEFI) and ofloxacin (OFLO) in combined tablet dosage form. The utility of dual wavelength data processing program is its ability to calculate unknown concentration of components of interest in a mixture containing an interfering component. The principle for dual wavelength method is “the absorbance difference between two points on the mixture spectra is directly proportional to the concentration of the component of interest”. The method was based on determination of ofloxacin at 350 nm using its absorptivity value and cefixime at 264 nm after deduction of absorbance due to ofloxacin. The two drugs follow Beer-Lanbert’s law over the concentration range of 2-14 µg/ml. The method was successfully applied to pharmaceutical dosage form because no interference from the tablet excipients was found. The results of analysis have been validated statistically and by recovery studies.
Authors and Affiliations
Satish Patel , Natavarlal Patel
Keywords
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