Development and Validation of High Performance LCMS Methods for Estimation of Ebastine and Carebastine in Human Plasma
Journal Title: International Journal of Pharmaceutical Sciences and Drug Research - Year 2018, Vol 10, Issue 6
Abstract
A simple and specific method for simultaneous determination of Ebastine and Carebastine by liquid chromatography-tandem mass spectrometry operated in positive ionization mode was developed and validated. The column used was a BDS Hypersil C18, 50 mm × 4.6 mm, 5µm with a flow rate of 0.6 mL/min, without splitter for the chromatographic analysis with flow rate of 0.6 mL/min. Solid phase extraction method was applied. Mass parameters, 496.2/261.0 and 672.2/479.3/261.2 were chosen for analysis. Linearity was established in human plasma covering the concentration range of 0.051 ng/mL to 31.099 ng/mL for Ebastine and 1.013 ng/mL to 1005.451 ng/mL for Carebastine using (Ebastine D6 & Carebastine D6) as internal standards. Different parameters such as linearity, range, precision, accuracy, ruggedness and robustness, limit of detection (LOD) and limit of quantification (LOQ) were used for full validation of the method. The results were found to be acceptable as per the guidelines of International Conference on Harmonization (ICH). The method found to be novel, rapid, linear, precise, accurate, robust and rugged and can be successfully applied for the routine analysis of Ebastine and Carebastine with more sensitivity and covers wider range of quantitation. The method also found to be useful and economical.
Authors and Affiliations
Sheeba Manoj Nair, Ravi Kumar P, Balasubramani Govindan, Sanjay . , Denish Karia
Keywords
Related Articles
- EP ID EP415878
- DOI 10.25004/IJPSDR.2018.100601
- Views 114
- Downloads 0
PHARMACOGNOSTICAL EVALUATION OF ROOTS OF SIMMONDSIA CHINENSIS SCHNEIDER
Simmondsia chinensis Schneider commonly known as jojoba is a semi-arid evergreen shrub. Jojoba is unique among plants in the fact that its products (seeds) contain about 50 percent by weight oil. Jojoba liquid wax genera...
AN OVERVIEW OF PHYTOTHERAPEUTIC APPROACH IN PREVENTION AND TREATMENT OF ALZHEIMER’S SYNDROME & DEMENTIA
Alzheimer disease (AD) is the most common form of the dementia which occurs among older people above the age of 60 years. The Alzheimer’s disease once considered a rare disorder and it is now seen as a major public healt...
THE RELATIONSHIP BETWEEN MAIN POLYPHENOL COMPONENTS AND FREE RADICAL SCAVENGING ACTIVITY OF SELECTED MEDICINAL PLANTS
This study investigates the relationships between the main polyphenols and DPPH radical scavenging activity (RSA) in extracts from some of the most common wild and cultivated species from the Achillea millefolium group,...
EFFECT OF CENTELLA ASIATICA (LINN) LEAVES ON AMINOTRANSFERASES IN STREPTOZOTOCIN INDUCED DIABETIC RATS
This study was conducted to evaluate the therapeutic effect of methanol extract Centella asiatica leaves by assaying the activities of diagnostic markers (aminotransferases) in the streptozotocin induced diabetic rats. T...
THERAPEUTIC ACTIVITY OF POLYHERBAL FORMULATION AGAINST PASSIVE ANAPHYLAXIS BY USING RAT MESENTERIC MAST CELLS
Anaphylaxis is the result of alteration in mast cell physiology and is responsible for the various physiological changes which are observed during anaphylaxis. The symptoms of anaphylaxis are due to the release of the hi...