DEVELOPMENT AND VALIDATION OF HIGH PERFORMANCE THIN LAYER CHROMATOGRAPHY FOR ESTIMATION OF MEFENAMIC ACID, PARACETAMOL AND DICYCLOMINE HCL IN TABLET DOSAGE FORM
Journal Title: International Journal of Pharmaceutical Sciences and Drug Research - Year 2015, Vol 7, Issue 4
Abstract
High performance thin layer chromatographic method was described for the analysis of mefenamic acid, paracetamol and dicyclomine HCl in tablet dosage form. The method employed HPTLC aluminium plates precoated with Silica gel 60F254 as the stationary phase and mixture of Toluene: Acetone: Formic acid (10:9.8:0.2) as mobile Phase. The densitometric evaluation of separated bands was carried out at 254 nm for mefenamic acid and paracetamol. In method development, Dicyclomine HCl was not detected in UV absorbance at 254 nm, so that same plate was poured in dragandroff reagent and measured at 510 nm wavelength. The linear response was observed in range of 100-700 ng/spot with correlation coefficient of, 0.998, 0.997 and 0.978. Precision was found to be within limits. The percentage recovery was found to be the limits of acceptance criteria between the range of 99.03% to 101.24%.
Authors and Affiliations
Twinkle K. Patel, Dhananjay B. Meshram
Keywords
Related Articles
- EP ID EP625080
- DOI -
- Views 74
- Downloads 0
FORMULATION AND EVALUATION OF NASAL NIOSOMAL IN SITU GELS OF LORATADINE
Loratadine is an antihistaminic drug, used in the treatment of allergic inflammation. Poor bioavailability of the drug from conventional dosage forms is especially attributable to mucociliary clearance and transient resi...
ANTI HYPERLIPIDEMIC POTENTIAL OF POLYHERBAL FORMULATION IN WISTAR ALBINO RATS
The present study aimed that evaluation of anti hyperlipidemic activity of Momordica charantia, stem and root of Tinospora cordifolia, whole plant of Andrographis paniculata and wood of Pterocarpus marsupium and leaves o...
DETERMINATION OF BIOACTIVE COMPONENTS OF ETHYL ACETATE FRACTION OF PUNICA GRANATUM RIND EXTRACT
Punica granatum belongs to a Punicaceae family. The Punica granatum is valued as a powerful medicinal plant and used in folk medicines. Hence the present investigation was carried out to determine the possible chemical c...
DEVELOPMENT AND VALIDATION OF STABILITY-INDICATING HPTLC METHOD FOR SIMULTANEOUS DETERMINATION OF TELMISARTAN AND CILNIDIPINE IN COMBINED TABLET DOSAGE FORM
A new simple, accurate, precise and selective stability-indicating high performance thin layer chromatographic (HPTLC) method has been developed and validated for simultaneous estimation of Telmisartan and Cilnidipine in...
DEVELOPMENT AND VALIDATION OF SIMPLE UV SPECTROPHOTOMETRIC METHOD FOR THE ESTIMATION OF DEXTROMETHORPHAN HYDROBROMIDE IN BULK AND MARKETED DOSAGE FORMULATIONS
A simple, rapid and economic UV spectrophotometric method has been developed and validated using a solvent 0.1N HCl to determine Dextromethorphan hydrobromide content in bulk and two different pharmaceutical solid dosage...