DEVELOPMENT AND VALIDATION OF HPLC METHOD FOR ESTIMATION OF BRIMONIDINE TARTRATE AS BULK DRUG AND IN OPHTHALMIC FORMULATION
Journal Title: Journal of Drug Delivery and Therapeutics - Year 2017, Vol 7, Issue 7
Abstract
The optimized reverse phase high performance liquid chromatographic method was developed for estimation of Brimonidine Tartrate in bulk drug and pharmaceutical dosage form. Chromatography was performed on Kromasil C 18 ( 250 mm X 4.6 mm i.d. , 5 μm particle size) column with mobile phase citric acid monohydrate buffer:water:methanol (30:50:20 v/v/v) and pH 3 was maintained by using triethylamine. The flow rate was 1.0 ml/min. Elute was detected at 246 nm and it effectively separated at Retention Time of 5.96 min. The LOD and LOQ was 1.47 and 4.47 μg/ml respectively. A linear response was observed over the concentration range 40-80 μg/ml for Brimonidine Tartrate. Thus the proposed HPLC method was found accurate, specific, precise, robust and reproducible.
Authors and Affiliations
S S Mandan
Keywords
Related Articles
- EP ID EP300621
- DOI -
- Views 80
- Downloads 0
FORMULATION AND EVALUATION OF HERBAL LOZENGES
Throat infections are most common disease in today’s world. However, it is not taken too seriously by people. Long term throat infection can lead to severe throat problems like Pharyngitis and also cancer. Lozenges are s...
FLAXSEEDS AND AJWAIN FOR PRIMARY HYPERLIPIDEMIA
Research work was single blind placebo-controlled, conducted at General Hospital, Lahore, Pakistan conducted from NOVEMBER 2015 to JANUARY 2016. 75 diagnosed primary hyperlipidemic patients were selected with age range...
DESIGN AND DEVELOPMENT OF CONTROLLED POROSITY OSMOTIC TABLETS OF GARCINIA INDICA EXTRACT
The present study deals with the development and evaluation of controlled porosity osmotic pump (CPOP) tablets containing inclusion complexes of ethanolic extract of Garcinia indica fruit rind. Wet granulation method was...
A SHORT COMPILATION ON ZIKA VIRUS TRANSMISSION AND ITS COMPLICATION DURING PREGNANCY
Zika virus, a mosquito borne flavivirus transmitted primarÂily by Aedes aegypti mosquitoes is a pathogen affecting humans. These vectors also transÂmit dengue and chikungunya virus and are found throughout much of the...
DIALLYLDISULFIDE CONTAINING POLYMERIC NANOPARTICLES FOR SITE-SPECIFIC DELIVERY IN COLON CANCER
Effective delivery of drugs to colon is a challenge, as the drug needs to be protected from gastrointestinal environment and should be released intact in colon. Present study is aimed to develop a site-specific nanopart...