DEVELOPMENT AND VALIDATION OF ISRADIPINE IN BULK AND IN ITS PHARMACEUTICAL FORMULATION BY RP-HPLC METHOD
Journal Title: International Research Journal of Pharmacy (IRJP) - Year 2012, Vol 3, Issue 9
Abstract
A simple, sensitive and selective RP-HPLC method for the estimation of Isradipine in pharmaceutical formulation was developed and validate in the present work. Chromatographic separation of drug is performed with kromasil c18 column having internal diameter 100mmX4.6 mm and 5µm diameter and the mobile phase consisting of a mixture of water , methanol and tetrahydrofuran(50:40:10, v/v/v), Isocratic elution at a flow rate of 1.4 ml/min with UV detection at 330nm at 25˚c is used in this method. The proposed RP-HPLC method is successfully applied for the determination of isradipine in pharmaceutical dosage form. The validation studies are carried out and it’s fulfilling ICH requirements.The method is found to be specific, linear, precise (including both intra- and inter- day precision), accurate and robust. The proposed method was successfully applied for the quantitative determination of Isradipine in tablet formulation.
Authors and Affiliations
Laxmi Aswini, D. Dachinamoorthy. Ravi Babu, Lakshmi Surekha, Kumara Swamy
Keywords
Related Articles
- EP ID EP114416
- DOI -
- Views 118
- Downloads 0
DIURETIC ACTIVITY OF BUTEA MONOSPERMA FLOWERS EXTRACT
Kidney, as excretory organ of our body serves important function of excretion of waste products, regulation of fluid volume and electrolyte content etc. Damage to kidney can lead to severe life threatening complications....
ETHNOBOTANICAL UTILIZATION OF SOME MEDICINAL PLANTS BY BODO PEOPLE OF MANAS BIOSPHERE RESERVE IN THE TREATMENT OF MALARIA
Malaria is a parasitic disease caused by Plasmodium falciparum and P. vivax, transmitted by a female Anopheles mosquito from the blood of an infected person to a healthy person. Malaria is one of the major problem in No...
DEVELOPMENT AND VALIDATION OF SIMULTANEOUS EQUATION METHOD FOR THE ESTIMATION OF CEFIXIME AND OFLOXACIN IN PURE AND IN TABLET FORMULATION
This paper describes developed and validated UV spectrophotometry method for the simultaneous estimation of Ofloxacin and Cefixime in a combined dosage form by Simultaneous equation method. This method utilize methanol a...
COMPARATIVE STUDY OF OCIMUM SANCTUM OIL AND ANTIMALARIAL TABLETS CONTAINING CHLOROQUINE PHOSPHATE
In traditional system of medicine, Ocimum sanctum (known as Tulsi in Hindi), have been recommended for treatment of many diseases such as malaria, diarrhoea, eye diseases, cold disease etc. In this study, the Ocimum sanc...
MICRONEEDLE AS A NOVEL DRUG DELIVERY SYSTEM: A REVIEW
Patch-based transdermal drug delivery offers a convenient way to administer drugs without the drawbacks of standard hypodermic injections relating to issues such as patient acceptability and injection safety. However, co...