Development and Validation of RP-HPLC Method for Simultaneous Estimation of Α- Lipoic Acid and Metformin in their Tablet Dosage Form
Journal Title: International Journal of Ayurveda and Pharmaceutical Chemistry - Year 2017, Vol 0, Issue 0
Abstract
The combination of Alpha- Lipoic Acid and Metformin HCl is used in treatment of Diabetes. The present work involves the development and validation of RP-HPLC method for the estimation of α- Lipoic Acid (ALA) and Metformin HCl (MET) in tablet dosage form. A specific, precise and selective RP-HPLC method was developed and validated for the estimation of α- Lipoic Acid and Metformin HCl in Tablet dosage form using Zodiac C18 (150mm x 4.6mm i.d., 5μm). Phosphate buffer: Acetonitrile (60:40) was selected as mobile phase. Flow rate was selected as 1.0 ml/min and detection was carried out at 220 nm. The retention time of α- Lipoic Acid and Metformin HCl was 8.983 min and 2.003 min was obtained respectively. The linearity range was found to be 2-12μg/ml and 150-500 μg/ml for α- Lipoic Acid and Metformin HCl respectively. The Correlation Coefficient r 2 = 0.9984 and r2 = 0.9989 was obtained for α- Lipoic Acid and Metformin HCl respectively. The mean recovery was found to be 99.20±1.14 and 99.28± 0.70 for α- Lipoic Acid and Metformin HCl respectively. The developed method was economic, precise, specific and validated as per ICH Guidelines.
Authors and Affiliations
Patel PK, Modha NB, Upadhyay UM
Keywords
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